Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 87790..88391 Replicon plasmid unnamed1
Accession NZ_CP033847
Organism Escherichia coli strain FDAARGOS_497

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag EGY17_RS00610 Protein ID WP_001216034.1
Coordinates 87790..88170 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag EGY17_RS00615 Protein ID WP_001190712.1
Coordinates 88170..88391 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY17_RS00575 82841..83773 - 933 WP_000081352.1 homocysteine S-methyltransferase -
EGY17_RS00580 83760..85163 - 1404 WP_001373486.1 S-methylmethionine permease -
EGY17_RS00585 85407..86387 + 981 WP_000019407.1 IS5-like element IS5 family transposase -
EGY17_RS00590 86615..86932 + 318 WP_001513659.1 hypothetical protein -
EGY17_RS00595 86882..87295 - 414 Protein_108 DDE-type integrase/transposase/recombinase -
EGY17_RS00600 87219..87578 - 360 WP_001513660.1 hypothetical protein -
EGY17_RS00605 87606..87785 - 180 WP_001513661.1 hypothetical protein -
EGY17_RS00610 87790..88170 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EGY17_RS00615 88170..88391 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EGY17_RS00620 88574..90130 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
EGY17_RS00625 90127..91410 + 1284 WP_001553855.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) - 1..143865 143865


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T114482 WP_001216034.1 NZ_CP033847:c88170-87790 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T114482 NZ_CP033847:c88170-87790 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT114482 WP_001190712.1 NZ_CP033847:c88391-88170 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT114482 NZ_CP033847:c88391-88170 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References