Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 73139..73698 Replicon plasmid unnamed1
Accession NZ_CP033847
Organism Escherichia coli strain FDAARGOS_497

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag EGY17_RS00500 Protein ID WP_000421272.1
Coordinates 73423..73698 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag EGY17_RS00495 Protein ID WP_001387467.1
Coordinates 73139..73423 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY17_RS00465 68602..69042 - 441 Protein_86 zinc-binding dehydrogenase -
EGY17_RS00475 69443..70333 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
EGY17_RS00480 70358..70738 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
EGY17_RS00485 70771..71736 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
EGY17_RS00490 71782..72534 - 753 WP_000562172.1 IclR family transcriptional regulator -
EGY17_RS00495 73139..73423 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
EGY17_RS00500 73423..73698 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGY17_RS00505 73814..74511 - 698 Protein_93 IS1 family transposase -
EGY17_RS00510 74581..74874 + 294 WP_001105060.1 hypothetical protein -
EGY17_RS00515 74909..75037 - 129 Protein_95 IS481 family transposase -
EGY17_RS00520 75070..76239 - 1170 WP_001072355.1 hypothetical protein -
EGY17_RS00530 76732..77039 + 308 Protein_97 type II toxin-antitoxin system ParD family antitoxin -
EGY17_RS00540 77382..77564 + 183 WP_042065278.1 hypothetical protein -
EGY17_RS00545 77685..78425 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) - 1..143865 143865


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T114481 WP_000421272.1 NZ_CP033847:73423-73698 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T114481 NZ_CP033847:73423-73698 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT114481 WP_001387467.1 NZ_CP033847:73139-73423 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT114481 NZ_CP033847:73139-73423 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References