Detailed information of TA system TA114478

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4204373..4205065
• Replicon: chromosome
• Accession: NZ_CP033844
• Organism: Klebsiella oxytoca strain FDAARGOS_500

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: EGY21_RS20320
• Protein ID: WP_102804018.1
• Coordinates: 4204715..4205065 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EGY21_RS20315
• Protein ID: WP_142385465.1
• Coordinates: 4204373..4204690 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY21_RS20280	4199853..4200590	-	738	WP_047935074.1	hypothetical protein	-
EGY21_RS20285	4200942..4201199	-	258	WP_102804013.1	IS66 family insertion sequence element accessory protein TnpB	-
EGY21_RS28125	4201247..4201546	-	300	Protein_3885	transposase	-
EGY21_RS20295	4202285..4203106	+	822	WP_102804014.1	DUF945 domain-containing protein	-
EGY21_RS20300	4203139..4203588	+	450	WP_102804015.1	antirestriction protein	-
EGY21_RS20305	4203601..4204080	+	480	WP_047724681.1	DNA repair protein RadC	-
EGY21_RS20310	4204094..4204315	+	222	WP_102804016.1	DUF987 domain-containing protein	-
EGY21_RS20315	4204373..4204690	+	318	WP_142385465.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EGY21_RS20320	4204715..4205065	+	351	WP_102804018.1	TA system toxin CbtA family protein	Toxin
EGY21_RS20325	4205159..4206401	+	1243	Protein_3892	AAA family ATPase	-
EGY21_RS20330	4206550..4207284	+	735	WP_123830109.1	TIGR02646 family protein	-
EGY21_RS20335	4207432..4208265	+	834	WP_123830110.1	DUF4942 domain-containing protein	-
EGY21_RS20340	4208409..4209206	+	798	WP_123830111.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4156722..4211208	54486
	-	flank	IS/Tn	-	-	4200942..4201199	257

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13220.22 Da
• Isoelectric Point: 8.1282

>T114478 WP_102804018.1 NZ_CP033844:4204715-4205065 [Klebsiella oxytoca]
MKTLPANQWVAKPCPPPVLVWQTLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGTTLADAVNFLVEKYELVRIDRRGFN
CQEQSPYLRAVDILRARQATGLLTQCKKRKCNQPDG

Nucleotide

• Length: 351 bp

>T114478 NZ_CP033844:4204715-4205065 [Klebsiella oxytoca]
ATGAAAACTCTTCCTGCAAATCAGTGGGTGGCGAAGCCATGCCCGCCGCCTGTGCTTGTCTGGCAAACGCTACTTACGCG
TCTGCTTGAGCAACACTATGGCCTGACGCTGAACGACACGCCGTTCAGTGATGAAACCGTTATTCAGGAGCATATCAACG
CCGGTACCACGCTGGCTGATGCCGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTTAAC
TGTCAGGAGCAGTCACCTTATCTTCGGGCTGTAGATATTTTACGGGCAAGGCAGGCAACCGGTTTGCTAACTCAGTGCAA
AAAACGCAAATGTAATCAGCCAGATGGTTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12010.72 Da
• Isoelectric Point: 6.9462

>AT114478 WP_142385465.1 NZ_CP033844:4204373-4204690 [Klebsiella oxytoca]
MSEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANFDGQFCDADLRHLDQAFPVLMKQLELMLTSGELNPRHLHCVTLYA
RGLTCEADTLGSHGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT114478 NZ_CP033844:4204373-4204690 [Klebsiella oxytoca]
ATGAGCGAACCCTGGTGGGGACTCAGGCGCAGTGTTTCCCCGTGTTTTGGTGCCCGCCTGGTCCAGGAGGGAAACCGCCT
GCATTACCTGGCTGACCGTGCGAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATCCACGGCACCTGCATTGTGTCACGTTGTATGCC
AGAGGGCTGACCTGCGAGGCCGATACGCTGGGTTCGCACGGCTACGTTTATATCGCTATCTATCCCACTCAACGTTAA