Detailed information of TA system TA114477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2869488..2870080
• Replicon: chromosome
• Accession: NZ_CP033844
• Organism: Klebsiella oxytoca strain FDAARGOS_500

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: EGY21_RS13835
• Protein ID: WP_016808034.1
• Coordinates: 2869488..2869862 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A2J4YM08
• Locus tag: EGY21_RS13840
• Protein ID: WP_004105957.1
• Coordinates: 2869859..2870080 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY21_RS13815	2864837..2866180	-	1344	WP_123830009.1	glycoside-pentoside-hexuronide (GPH):cation symporter	-
EGY21_RS13820	2866288..2867154	+	867	WP_123830010.1	AraC family transcriptional regulator	-
EGY21_RS13825	2867283..2868926	+	1644	WP_053064444.1	glycoside hydrolase family 43 protein	-
EGY21_RS13830	2869005..2869508	+	504	WP_004105953.1	M48 family metallopeptidase	-
EGY21_RS13835	2869488..2869862	-	375	WP_016808034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
EGY21_RS13840	2869859..2870080	-	222	WP_004105957.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
EGY21_RS13845	2870240..2871229	+	990	WP_023330070.1	Gfo/Idh/MocA family oxidoreductase	-
EGY21_RS13850	2871480..2872448	+	969	WP_004105962.1	TerC family protein	-
EGY21_RS13855	2872703..2873950	+	1248	WP_004105963.1	serine/threonine transporter SstT	-
EGY21_RS13860	2873965..2874519	-	555	WP_004105965.1	YgjV family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13835.89 Da
• Isoelectric Point: 6.0752

>T114477 WP_016808034.1 NZ_CP033844:c2869862-2869488 [Klebsiella oxytoca]
MKWVSASEVIAFHDRILQHLPGVKGMSDPGRAEAIIYRVQNRFHCEGVNDIFELAATYWVAIARGHIFNDGNKRTAFFIT
MTFLARNGYLIADEDTRLEELTVLAATGEATVVVLADALRQLAL

Nucleotide

• Length: 375 bp

>T114477 NZ_CP033844:c2869862-2869488 [Klebsiella oxytoca]
ATGAAATGGGTGAGCGCAAGCGAAGTCATTGCCTTTCATGATCGCATTTTACAACATCTCCCCGGCGTTAAAGGTATGTC
CGATCCTGGTCGGGCGGAAGCCATAATTTATCGTGTACAGAACCGCTTTCACTGTGAAGGGGTGAACGACATTTTTGAAC
TGGCTGCAACCTATTGGGTCGCTATCGCGCGTGGGCACATTTTTAATGATGGTAATAAGAGGACTGCTTTTTTTATCACC
ATGACGTTCCTGGCCCGCAATGGGTATCTTATCGCTGATGAAGATACCCGGTTGGAAGAACTCACTGTGCTGGCTGCGAC
AGGCGAAGCTACGGTAGTAGTTCTTGCTGATGCGTTACGGCAATTGGCGCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8185.19 Da
• Isoelectric Point: 5.7996

>AT114477 WP_004105957.1 NZ_CP033844:c2870080-2869859 [Klebsiella oxytoca]
MRSYSTSEARQNIAAVMDAAASGEPIEITRRDGTSNVLISKAEYETFMNAKLDAEFDFIMQRHGRTVKALTDR

Nucleotide

• Length: 222 bp

>AT114477 NZ_CP033844:c2870080-2869859 [Klebsiella oxytoca]
ATGCGCTCTTATAGTACATCTGAAGCCAGACAGAATATTGCGGCCGTCATGGACGCTGCTGCCAGCGGAGAACCGATTGA
AATTACGCGGCGTGATGGAACGTCTAATGTTTTGATCAGTAAGGCTGAATATGAAACGTTTATGAATGCCAAACTTGATG
CGGAGTTTGATTTTATTATGCAGCGACACGGCCGCACAGTTAAGGCGTTGACCGATCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4YM08