Detailed information of TA system TA114476

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2613007..2613664
• Replicon: chromosome
• Accession: NZ_CP033844
• Organism: Klebsiella oxytoca strain FDAARGOS_500

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: EGY21_RS12525
• Protein ID: WP_064370303.1
• Coordinates: 2613007..2613417 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A285B945
• Locus tag: EGY21_RS12530
• Protein ID: WP_004105561.1
• Coordinates: 2613398..2613664 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY21_RS12505	2608984..2610717	-	1734	WP_004105553.1	single-stranded-DNA-specific exonuclease RecJ	-
EGY21_RS12510	2610723..2611436	-	714	WP_123829948.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
EGY21_RS12515	2611459..2612355	-	897	WP_004105555.1	site-specific tyrosine recombinase XerD	-
EGY21_RS12520	2612477..2612998	+	522	WP_004105557.1	flavodoxin FldB	-
EGY21_RS12525	2613007..2613417	-	411	WP_064370303.1	protein YgfX	Toxin
EGY21_RS12530	2613398..2613664	-	267	WP_004105561.1	FAD assembly factor SdhE	Antitoxin
EGY21_RS12535	2613908..2614891	+	984	WP_004105567.1	tRNA-modifying protein YgfZ	-
EGY21_RS12540	2615067..2617865	+	2799	WP_023321613.1	transcriptional regulator DagR	-
EGY21_RS12545	2617986..2618411	+	426	WP_004854067.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16091.86 Da
• Isoelectric Point: 10.9455

>T114476 WP_064370303.1 NZ_CP033844:c2613417-2613007 [Klebsiella oxytoca]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLVLPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIVGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDTGEWRDLRRLVLQKPTQD

Nucleotide

• Length: 411 bp

>T114476 NZ_CP033844:c2613417-2613007 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCAGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGTCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACACGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10555.02 Da
• Isoelectric Point: 5.1498

>AT114476 WP_004105561.1 NZ_CP033844:c2613664-2613398 [Klebsiella oxytoca]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDDDKRLFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT114476 NZ_CP033844:c2613664-2613398 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A285B945