Detailed information of TA system TA114475

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2143654..2144437
• Replicon: chromosome
• Accession: NZ_CP033844
• Organism: Klebsiella oxytoca strain FDAARGOS_500

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EGY21_RS10205
• Protein ID: WP_048225456.1
• Coordinates: 2144063..2144437 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EGY21_RS10200
• Protein ID: WP_123829884.1
• Coordinates: 2143654..2144013 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY21_RS10155	2138870..2139727	+	858	WP_123830341.1	hypothetical protein	-
EGY21_RS10160	2140005..2140475	+	471	WP_123829879.1	hypothetical protein	-
EGY21_RS10165	2140530..2140889	+	360	WP_004136901.1	hypothetical protein	-
EGY21_RS10170	2140975..2141187	+	213	WP_032755286.1	hypothetical protein	-
EGY21_RS10175	2141294..2142115	+	822	WP_123829880.1	DUF945 domain-containing protein	-
EGY21_RS10180	2142194..2142673	+	480	WP_123829881.1	antirestriction protein	-
EGY21_RS10185	2142684..2142917	+	234	WP_123829882.1	hypothetical protein	-
EGY21_RS10190	2142914..2143396	+	483	WP_123829883.1	DNA repair protein RadC	-
EGY21_RS10195	2143410..2143631	+	222	WP_032755299.1	DUF987 domain-containing protein	-
EGY21_RS10200	2143654..2144013	+	360	WP_123829884.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EGY21_RS10205	2144063..2144437	+	375	WP_048225456.1	TA system toxin CbtA family protein	Toxin
EGY21_RS10210	2144517..2144837	-	321	WP_049848451.1	hypothetical protein	-
EGY21_RS10215	2144976..2145965	-	990	WP_123829885.1	glycosyltransferase	-
EGY21_RS10220	2146806..2148044	+	1239	WP_123829886.1	IS110 family transposase	-
EGY21_RS10225	2148229..2149137	-	909	WP_032938037.1	kdo(2)-lipid IV(A) palmitoleoyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2127913..2169725	41812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (8-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13974.16 Da
• Isoelectric Point: 10.3161

>T114475 WP_048225456.1 NZ_CP033844:2144063-2144437 [Klebsiella oxytoca]
MQIHSLSLQRAASSRLSSVEIWRKLLKYLLKQHYGLMLNDTPFGNDEVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGF
SVKEQSPFIGSIDMLRARKATGLMTRKGYKTVTDITGGRFSGGK

Nucleotide

• Length: 375 bp

>T114475 NZ_CP033844:2144063-2144437 [Klebsiella oxytoca]
ATGCAAATACATTCCCTGTCCCTTCAAAGGGCGGCATCGTCACGTCTGTCGTCAGTCGAAATCTGGCGAAAATTACTGAA
GTATCTGCTGAAACAACACTACGGTCTGATGCTGAACGACACACCGTTTGGCAACGACGAAGTGATCCAGAAGCACATTG
ATGCGGGAATCTCACTGTGTGACGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGTAGCATTGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13498.62 Da
• Isoelectric Point: 7.3239

>AT114475 WP_123829884.1 NZ_CP033844:2143654-2144013 [Klebsiella oxytoca]
MQKTTRVINHNITEPWWGLRRNITPCFGARLVQEGNHLHYLADRASIAGTFNDADLRHLDQVFPVLMKQMELMLANSELT
PRIQRCITLHVKGLICEADTLGSCGYLYIVIYPASATTE

Nucleotide

• Length: 360 bp

>AT114475 NZ_CP033844:2143654-2144013 [Klebsiella oxytoca]
ATGCAGAAAACAACTCGTGTAATCAACCATAACATCACGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
TGGTGCACGGCTGGTACAGGAGGGTAACCACCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGTATTTCCGGTACTGATGAAGCAGATGGAGCTTATGCTGGCCAACAGCGAACTCACG
CCCCGCATTCAGCGCTGCATCACGCTCCACGTAAAAGGGCTGATATGTGAGGCCGATACACTCGGCTCCTGCGGTTATTT
GTACATCGTAATTTATCCGGCGTCTGCAACAACCGAATAA