Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1034181..1034817 Replicon chromosome
Accession NZ_CP033841
Organism Bifidobacterium breve strain FDAARGOS_561

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag EGX97_RS04600 Protein ID WP_003829234.1
Coordinates 1034181..1034537 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag EGX97_RS04605 Protein ID WP_012577921.1
Coordinates 1034524..1034817 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX97_RS04570 1029372..1030283 + 912 WP_003829224.1 ABC transporter ATP-binding protein -
EGX97_RS04575 1030480..1031106 + 627 WP_003829226.1 30S ribosomal protein S4 -
EGX97_RS04580 1031443..1032225 - 783 WP_003829227.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
EGX97_RS04585 1032366..1032851 - 486 WP_003829229.1 cytidine deaminase -
EGX97_RS04590 1032941..1033297 + 357 WP_003829232.1 SdpI family protein -
EGX97_RS04595 1033402..1034004 + 603 WP_003829233.1 transglutaminase family protein -
EGX97_RS04600 1034181..1034537 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EGX97_RS04605 1034524..1034817 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EGX97_RS04610 1034968..1035132 - 165 Protein_852 IS30 family transposase -
EGX97_RS04615 1035232..1035906 + 675 WP_032738178.1 histidine phosphatase family protein -
EGX97_RS04620 1035989..1036393 + 405 WP_003829238.1 DUF948 domain-containing protein -
EGX97_RS04625 1036393..1036629 + 237 WP_003829239.1 hypothetical protein -
EGX97_RS04630 1036725..1039403 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T114460 WP_003829234.1 NZ_CP033841:c1034537-1034181 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T114460 NZ_CP033841:c1034537-1034181 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT114460 WP_012577921.1 NZ_CP033841:c1034817-1034524 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT114460 NZ_CP033841:c1034817-1034524 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References