Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 974869..975424 Replicon chromosome
Accession NZ_CP033841
Organism Bifidobacterium breve strain FDAARGOS_561

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag EGX97_RS04315 Protein ID WP_003829109.1
Coordinates 974869..975192 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag EGX97_RS04320 Protein ID WP_003829112.1
Coordinates 975179..975424 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX97_RS04280 969906..970194 - 289 Protein_790 IS256 family transposase -
EGX97_RS04290 970665..971930 - 1266 WP_053001023.1 Fic family protein -
EGX97_RS04300 972472..972837 - 366 WP_032738175.1 YccF domain-containing protein -
EGX97_RS04305 973075..973944 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
EGX97_RS04310 974038..974652 + 615 WP_003829107.1 cupin domain-containing protein -
EGX97_RS04315 974869..975192 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EGX97_RS04320 975179..975424 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EGX97_RS04325 975598..976854 + 1257 WP_003829116.1 HipA domain-containing protein -
EGX97_RS04330 977119..977310 + 192 WP_003829118.1 hypothetical protein -
EGX97_RS04335 977494..978765 - 1272 WP_003829121.1 MFS transporter -
EGX97_RS04340 978922..979584 - 663 WP_025262926.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 964638..1012923 48285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T114459 WP_003829109.1 NZ_CP033841:c975192-974869 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T114459 NZ_CP033841:c975192-974869 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT114459 WP_003829112.1 NZ_CP033841:c975424-975179 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT114459 NZ_CP033841:c975424-975179 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References