Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) abiEi-abiEii/AbiEii-AbiEi
Location 2018844..2020263 Replicon chromosome
Accession NZ_CP033837
Organism Fusobacterium necrophorum strain FDAARGOS_565

Toxin (Protein)


Gene name abiEi Uniprot ID A0A654RX45
Locus tag EGX98_RS09240 Protein ID WP_074517856.1
Coordinates 2019430..2020263 (+) Length 278 a.a.

Antitoxin (Protein)


Gene name abiEii Uniprot ID A0A654S274
Locus tag EGX98_RS09235 Protein ID WP_074517855.1
Coordinates 2018844..2019437 (+) Length 198 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX98_RS09220 2014871..2016859 + 1989 WP_074517892.1 C40 family peptidase -
EGX98_RS09225 2016871..2017161 + 291 WP_074517853.1 conjugal transfer protein -
EGX98_RS09230 2017166..2018752 + 1587 WP_124019684.1 DUF4366 domain-containing protein -
EGX98_RS09235 2018844..2019437 + 594 WP_074517855.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
EGX98_RS09240 2019430..2020263 + 834 WP_074517856.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
EGX98_RS09245 2020306..2020555 + 250 Protein_1785 HTH domain-containing protein -
EGX98_RS09250 2020662..2022374 + 1713 WP_074517857.1 DNA topoisomerase 3 -
EGX98_RS09255 2022378..2022482 + 105 Protein_1787 NAD(P)-dependent oxidoreductase -
EGX98_RS09260 2022619..2023596 + 978 WP_074517858.1 IS5 family transposase -
EGX98_RS09265 2023729..2024169 + 441 Protein_1789 NAD(P)-dependent oxidoreductase -
EGX98_RS09270 2024212..2024958 + 747 WP_074517859.1 conjugal transfer protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(33-266)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(6-50)


Sequences

Toxin        


Download         Length: 278 a.a.        Molecular weight: 32510.24 Da        Isoelectric Point: 6.1623

>T114456 WP_074517856.1 NZ_CP033837:2019430-2020263 [Fusobacterium necrophorum]
MNKDKLRAICQKLSKETGLSFNAVQTHYFLESILEKIASSDESENFIFKGGFLLANVIGIRQRNTVDIDFLIRRFSLTEE
NIKQRFEKILQSGNDNGITYEIQKIEEIRKEDEYGGFRITVLCRLENIRQIIPLDIATGDPITPSEISYEYKSIFSDKFF
EICAYNIETMLAEKIQTVYKRGAFNSRSKDFYDIYILYHLKKEEIDFEHLKEACKNTFKHRSTGFNIDSILDVLVDLKME
KDLKTRWSSYQKRFSYAGEINFDAVIDIMIELVKNIR

Download         Length: 834 bp

>T114456 NZ_CP033837:2019430-2020263 [Fusobacterium necrophorum]
ATGAATAAAGATAAGCTAAGAGCGATATGTCAAAAACTGTCGAAAGAAACAGGACTTAGCTTTAATGCTGTGCAAACACA
CTATTTTTTAGAAAGCATTTTAGAGAAAATAGCAAGTAGTGACGAGAGTGAAAACTTTATTTTTAAAGGTGGTTTTTTAC
TCGCAAATGTAATTGGAATAAGGCAGAGAAACACAGTAGATATTGATTTTTTGATAAGAAGATTTTCACTTACTGAAGAA
AACATCAAGCAAAGATTTGAAAAAATTCTTCAAAGCGGAAACGATAATGGAATTACTTATGAGATTCAAAAAATTGAAGA
AATCCGTAAAGAAGATGAATATGGAGGATTTAGAATTACAGTTCTTTGCAGACTTGAAAATATCAGGCAGATTATTCCAC
TCGATATAGCAACAGGCGATCCAATTACACCAAGTGAAATATCCTATGAATATAAAAGTATTTTTAGTGATAAGTTTTTT
GAAATTTGTGCATATAATATAGAAACAATGCTTGCTGAGAAGATTCAGACAGTATATAAAAGAGGGGCATTTAACAGCAG
AAGTAAGGATTTTTATGATATTTACATTCTATATCATTTGAAGAAAGAGGAAATAGACTTTGAGCATTTAAAGGAAGCTT
GTAAAAATACATTTAAGCATAGAAGCACAGGTTTTAATATAGATAGTATTCTTGATGTATTAGTCGACTTAAAAATGGAA
AAAGACCTCAAAACAAGGTGGAGTAGCTATCAAAAAAGATTTAGTTATGCAGGAGAAATAAATTTTGATGCTGTAATAGA
TATTATGATAGAATTAGTGAAAAACATAAGATAA

Antitoxin


Download         Length: 198 a.a.        Molecular weight: 23306.87 Da        Isoelectric Point: 8.5137

>AT114456 WP_074517855.1 NZ_CP033837:2018844-2019437 [Fusobacterium necrophorum]
MDYIKKIKKKLSKTGGVITSKELKNSNIPTIYLTRMVEKGELIRADRGIYIDSNGDYDEYYFFHNRCKVAVFSYVSALYL
QGFTDIIPNEMEVTVYKGYNTHRISGSVRIHYVTKEIYDLGMTECQTMFGNTVKVYDLERIICDFIKNRSEIETELLSKT
INKYVRYENKDLNKLYEYSKKMNIYEKVKEILEVVYE

Download         Length: 594 bp

>AT114456 NZ_CP033837:2018844-2019437 [Fusobacterium necrophorum]
ATGGATTATATAAAAAAGATAAAAAAAAAATTGAGTAAAACGGGTGGAGTTATAACAAGTAAGGAACTTAAAAACTCTAA
CATTCCTACAATATATCTCACACGAATGGTGGAAAAAGGTGAACTGATTCGTGCGGATAGAGGAATCTATATAGATTCAA
ACGGAGATTATGACGAGTATTATTTTTTTCATAATAGATGTAAGGTTGCTGTTTTTTCTTATGTATCCGCTCTTTATCTT
CAGGGGTTTACAGACATAATCCCGAATGAAATGGAAGTTACAGTATATAAAGGATACAATACTCATCGTATAAGTGGAAG
TGTAAGAATTCATTATGTAACAAAAGAAATTTATGATTTGGGTATGACAGAATGCCAAACAATGTTTGGGAATACAGTAA
AAGTTTATGACTTGGAGAGAATAATTTGCGATTTTATTAAAAATAGAAGTGAAATAGAAACAGAACTGCTTTCTAAAACG
ATTAACAAGTATGTCCGTTATGAAAATAAGGATTTGAATAAACTATATGAGTATTCTAAAAAAATGAATATATATGAAAA
AGTAAAAGAAATTTTGGAGGTTGTTTATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A654RX45

Antitoxin

Source ID Structure
AlphaFold DB A0A654S274

References