Detailed information of TA system TA114445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 6064270..6065312
• Replicon: chromosome
• Accession: NZ_CP033833
• Organism: Pseudomonas aeruginosa strain FDAARGOS_571

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EGY26_RS29600
• Protein ID: WP_003153636.1
• Coordinates: 6064737..6065312 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: EGY26_RS29595
• Protein ID: WP_003050245.1
• Coordinates: 6064270..6064740 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY26_RS29560	6059662..6061080	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
EGY26_RS29565	6061070..6061981	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
EGY26_RS29570	6061978..6062670	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
EGY26_RS29575	6062667..6063065	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
EGY26_RS29580	6063077..6063436	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
EGY26_RS29585	6063453..6063686	-	234	WP_006226027.1	TIGR03758 family integrating conjugative element protein	-
EGY26_RS29590	6063683..6064066	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
EGY26_RS29595	6064270..6064740	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
EGY26_RS29600	6064737..6065312	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
EGY26_RS29605	6065330..6066244	+	915	WP_016852809.1	AAA family ATPase	-
EGY26_RS29610	6066241..6066711	+	471	WP_003090160.1	hypothetical protein	-
EGY26_RS29615	6066708..6067208	+	501	WP_003090159.1	hypothetical protein	-
EGY26_RS29620	6067208..6068110	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
EGY26_RS29625	6068149..6068874	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5989695..6113708	124013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T114445 WP_003153636.1 NZ_CP033833:6064737-6065312 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T114445 NZ_CP033833:6064737-6065312 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT114445 WP_003050245.1 NZ_CP033833:6064270-6064740 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT114445 NZ_CP033833:6064270-6064740 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68