Detailed information of TA system TA114443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 3392988..3393493
• Replicon: chromosome
• Accession: NZ_CP033833
• Organism: Pseudomonas aeruginosa strain FDAARGOS_571

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: EGY26_RS16500
• Protein ID: WP_003083773.1
• Coordinates: 3392988..3393269 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: EGY26_RS16505
• Protein ID: WP_003083775.1
• Coordinates: 3393266..3393493 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY26_RS16475	3388239..3389588	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
EGY26_RS16480	3389637..3390323	+	687	WP_003083762.1	FadR family transcriptional regulator	-
EGY26_RS16485	3390424..3391158	+	735	WP_003083764.1	GntR family transcriptional regulator	-
EGY26_RS16490	3391362..3391748	+	387	WP_003083767.1	aegerolysin family protein	-
EGY26_RS16495	3391780..3392688	-	909	WP_003083769.1	LysR family transcriptional regulator	-
EGY26_RS16500	3392988..3393269	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
EGY26_RS16505	3393266..3393493	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
EGY26_RS16510	3393669..3394289	-	621	WP_003101226.1	hypothetical protein	-
EGY26_RS16515	3394390..3394890	+	501	WP_003083778.1	LEA type 2 family protein	-
EGY26_RS16520	3394963..3395304	+	342	WP_014603467.1	alkylphosphonate utilization protein	-
EGY26_RS16525	3395386..3396813	-	1428	WP_003083784.1	GABA permease	-
EGY26_RS16530	3396982..3398475	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T114443 WP_003083773.1 NZ_CP033833:c3393269-3392988 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T114443 NZ_CP033833:c3393269-3392988 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT114443 WP_003083775.1 NZ_CP033833:c3393493-3393266 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT114443 NZ_CP033833:c3393493-3393266 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGATGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9