Detailed information of TA system TA114437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4353937..4354979
• Replicon: chromosome
• Accession: NZ_CP033832
• Organism: Pseudomonas aeruginosa strain FDAARGOS_505

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EGY23_RS20940
• Protein ID: WP_003153636.1
• Coordinates: 4354404..4354979 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: EGY23_RS20935
• Protein ID: WP_003050245.1
• Coordinates: 4353937..4354407 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY23_RS20900	4349329..4350747	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
EGY23_RS20905	4350737..4351648	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
EGY23_RS20910	4351645..4352337	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
EGY23_RS20915	4352334..4352732	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
EGY23_RS20920	4352744..4353103	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
EGY23_RS20925	4353120..4353353	-	234	WP_006226027.1	TIGR03758 family integrating conjugative element protein	-
EGY23_RS20930	4353350..4353733	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
EGY23_RS20935	4353937..4354407	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
EGY23_RS20940	4354404..4354979	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
EGY23_RS20945	4354997..4355911	+	915	WP_016852809.1	AAA family ATPase	-
EGY23_RS20950	4355908..4356378	+	471	WP_003090160.1	hypothetical protein	-
EGY23_RS20955	4356375..4356875	+	501	WP_003090159.1	hypothetical protein	-
EGY23_RS20960	4356875..4357777	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
EGY23_RS20965	4357816..4358541	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4279362..4401095	121733

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T114437 WP_003153636.1 NZ_CP033832:4354404-4354979 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T114437 NZ_CP033832:4354404-4354979 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT114437 WP_003050245.1 NZ_CP033832:4353937-4354407 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT114437 NZ_CP033832:4353937-4354407 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68