Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 56277..56917 Replicon chromosome
Accession NZ_CP033832
Organism Pseudomonas aeruginosa strain FDAARGOS_505

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag EGY23_RS00315 Protein ID WP_003134109.1
Coordinates 56277..56687 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag EGY23_RS00320 Protein ID WP_031634724.1
Coordinates 56687..56917 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY23_RS00275 52403..52600 + 198 WP_019396765.1 hypothetical protein -
EGY23_RS00280 52757..53485 + 729 WP_004352842.1 TIGR03761 family integrating conjugative element protein -
EGY23_RS00285 53491..54039 + 549 WP_004352841.1 DUF3158 family protein -
EGY23_RS00290 54086..54925 + 840 WP_003105750.1 Rha family transcriptional regulator -
EGY23_RS00295 54955..55443 + 489 WP_004352840.1 single-stranded DNA-binding protein -
EGY23_RS00300 55599..55766 + 168 WP_128729268.1 CrpP family protein -
EGY23_RS00305 55832..56050 - 219 WP_023083218.1 hypothetical protein -
EGY23_RS00310 56064..56261 - 198 WP_023083219.1 hypothetical protein -
EGY23_RS00315 56277..56687 - 411 WP_003134109.1 tRNA(fMet)-specific endonuclease VapC Toxin
EGY23_RS00320 56687..56917 - 231 WP_031634724.1 antitoxin Antitoxin
EGY23_RS00325 57173..59092 + 1920 WP_004352838.1 type I DNA topoisomerase -
EGY23_RS00330 59400..59609 + 210 WP_003105733.1 cold-shock protein -
EGY23_RS00335 59830..61719 + 1890 WP_003105732.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 37920..127485 89565


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(7-33)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15452.78 Da        Isoelectric Point: 7.3233

>T114432 WP_003134109.1 NZ_CP033832:c56687-56277 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Download         Length: 411 bp

>T114432 NZ_CP033832:c56687-56277 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACTATCAAGAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGACACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8420.29 Da        Isoelectric Point: 4.2821

>AT114432 WP_031634724.1 NZ_CP033832:c56917-56687 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Download         Length: 231 bp

>AT114432 NZ_CP033832:c56917-56687 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGTTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References