Detailed information of TA system TA114432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 56277..56917
• Replicon: chromosome
• Accession: NZ_CP033832
• Organism: Pseudomonas aeruginosa strain FDAARGOS_505

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EGY23_RS00315
• Protein ID: WP_003134109.1
• Coordinates: 56277..56687 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EGY23_RS00320
• Protein ID: WP_031634724.1
• Coordinates: 56687..56917 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY23_RS00275	52403..52600	+	198	WP_019396765.1	hypothetical protein	-
EGY23_RS00280	52757..53485	+	729	WP_004352842.1	TIGR03761 family integrating conjugative element protein	-
EGY23_RS00285	53491..54039	+	549	WP_004352841.1	DUF3158 family protein	-
EGY23_RS00290	54086..54925	+	840	WP_003105750.1	Rha family transcriptional regulator	-
EGY23_RS00295	54955..55443	+	489	WP_004352840.1	single-stranded DNA-binding protein	-
EGY23_RS00300	55599..55766	+	168	WP_128729268.1	CrpP family protein	-
EGY23_RS00305	55832..56050	-	219	WP_023083218.1	hypothetical protein	-
EGY23_RS00310	56064..56261	-	198	WP_023083219.1	hypothetical protein	-
EGY23_RS00315	56277..56687	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EGY23_RS00320	56687..56917	-	231	WP_031634724.1	antitoxin	Antitoxin
EGY23_RS00325	57173..59092	+	1920	WP_004352838.1	type I DNA topoisomerase	-
EGY23_RS00330	59400..59609	+	210	WP_003105733.1	cold-shock protein	-
EGY23_RS00335	59830..61719	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	37920..127485	89565

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T114432 WP_003134109.1 NZ_CP033832:c56687-56277 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T114432 NZ_CP033832:c56687-56277 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACTATCAAGAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGACACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT114432 WP_031634724.1 NZ_CP033832:c56917-56687 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT114432 NZ_CP033832:c56917-56687 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGTTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA