Detailed information of TA system TA114404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 157423..158066
• Replicon: plasmid unnamed1
• Accession: NZ_CP033823
• Organism: Klebsiella sp. FDAARGOS_511

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EGY08_RS00855
• Protein ID: WP_016236302.1
• Coordinates: 157650..158066 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: EGY08_RS00850
• Protein ID: WP_001261282.1
• Coordinates: 157423..157653 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY08_RS00825	153139..153396	-	258	WP_023292103.1	hypothetical protein	-
EGY08_RS00830	154001..155455	+	1455	WP_074180203.1	EAL domain-containing protein	-
EGY08_RS00835	156073..156504	-	432	WP_174805835.1	hypothetical protein	-
EGY08_RS00845	157044..157466	-	423	WP_160887026.1	hypothetical protein	-
EGY08_RS00850	157423..157653	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EGY08_RS00855	157650..158066	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGY08_RS00860	158140..159702	+	1563	WP_004206609.1	AAA family ATPase	-
EGY08_RS00865	159687..160709	+	1023	WP_000361404.1	helicase UvrD	-
EGY08_RS00870	161254..162162	+	909	WP_032451458.1	HNH endonuclease	-
EGY08_RS00875	162348..162698	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..186836	186836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T114404 WP_016236302.1 NZ_CP033823:157650-158066 [Klebsiella sp. FDAARGOS_511]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T114404 NZ_CP033823:157650-158066 [Klebsiella sp. FDAARGOS_511]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT114404 WP_001261282.1 NZ_CP033823:157423-157653 [Klebsiella sp. FDAARGOS_511]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT114404 NZ_CP033823:157423-157653 [Klebsiella sp. FDAARGOS_511]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3