Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 26705..27441 Replicon plasmid unnamed1
Accession NZ_CP033823
Organism Klebsiella sp. FDAARGOS_511

Toxin (Protein)


Gene name tacT Uniprot ID J5UJ49
Locus tag EGY08_RS00140 Protein ID WP_009654334.1
Coordinates 26959..27441 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag EGY08_RS00135 Protein ID WP_003026799.1
Coordinates 26705..26971 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY08_RS00115 22705..23673 - 969 WP_077266818.1 IS5 family transposase -
EGY08_RS00120 23653..24684 + 1032 WP_064352057.1 IS110 family transposase -
EGY08_RS00125 25003..25971 - 969 WP_077267151.1 IS5 family transposase -
EGY08_RS00130 26032..26466 + 435 WP_042928448.1 cell envelope integrity protein TolA -
EGY08_RS00135 26705..26971 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
EGY08_RS00140 26959..27441 + 483 WP_009654334.1 GNAT family N-acetyltransferase Toxin
EGY08_RS00145 27648..28994 + 1347 WP_077266289.1 ISNCY family transposase -
EGY08_RS00150 29043..29441 + 399 WP_032422684.1 helix-turn-helix domain-containing protein -
EGY08_RS00155 29805..29939 - 135 Protein_29 IS5/IS1182 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..186836 186836
- inside IScluster/Tn - - 22705..40452 17747


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17339.06 Da        Isoelectric Point: 8.7400

>T114403 WP_009654334.1 NZ_CP033823:26959-27441 [Klebsiella sp. FDAARGOS_511]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHVKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYVHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T114403 NZ_CP033823:26959-27441 [Klebsiella sp. FDAARGOS_511]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATATCTCATTGCACGTAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGTTCACCATGGATTTAAGGCATCACAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT114403 WP_003026799.1 NZ_CP033823:26705-26971 [Klebsiella sp. FDAARGOS_511]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT114403 NZ_CP033823:26705-26971 [Klebsiella sp. FDAARGOS_511]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4QCA1

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References