Detailed information of TA system TA114400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3775363..3776102
• Replicon: chromosome
• Accession: NZ_CP033817
• Organism: Klebsiella aerogenes strain FDAARGOS_513

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: EGY11_RS18550
• Protein ID: WP_032705770.1
• Coordinates: 3775617..3776102 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: EGY11_RS18545
• Protein ID: WP_015366659.1
• Coordinates: 3775363..3775629 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY11_RS18525	3771656..3772273	+	618	WP_032705773.1	response regulator transcription factor	-
EGY11_RS18530	3772369..3772866	+	498	WP_015366656.1	heme-binding protein	-
EGY11_RS18535	3773267..3773800	+	534	WP_100280375.1	N-acetyltransferase	-
EGY11_RS18540	3773855..3775090	-	1236	WP_015366658.1	MFS transporter	-
EGY11_RS18545	3775363..3775629	+	267	WP_015366659.1	DUF1778 domain-containing protein	Antitoxin
EGY11_RS18550	3775617..3776102	+	486	WP_032705770.1	GNAT family N-acetyltransferase	Toxin
EGY11_RS18555	3776174..3777790	+	1617	WP_015366662.1	FAD-NAD(P)-binding protein	-
EGY11_RS18560	3777909..3779009	+	1101	WP_015366663.1	NADH:flavin oxidoreductase/NADH oxidase	-
EGY11_RS18565	3779066..3779224	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
EGY11_RS18570	3779485..3780555	+	1071	WP_015366664.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17573.23 Da
• Isoelectric Point: 8.8793

>T114400 WP_032705770.1 NZ_CP033817:3775617-3776102 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEESHQVVGFYSLATGSVNHSEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T114400 NZ_CP033817:3775617-3776102 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCACAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACTCGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTTTCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTGTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTACGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACGATTCTATTTACACCACGGATTCAAAGCCTCAACCACCCAGGAACGCACTCTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10075.52 Da
• Isoelectric Point: 4.6558

>AT114400 WP_015366659.1 NZ_CP033817:3775363-3775629 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANSALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT114400 NZ_CP033817:3775363-3775629 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCACGGACCGACTTTATTCTTGAGATGGCTTGCCAGGCGGCAGAAAACGTCATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACTCCGCTCTCGATAAATTGCTG
GCGAGAAAACCACAATGGGAAAAATAA