Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 1146474..1147495 Replicon chromosome
Accession NZ_CP033817
Organism Klebsiella aerogenes strain FDAARGOS_513

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A0G9UXE3
Locus tag EGY11_RS05640 Protein ID WP_032706547.1
Coordinates 1146920..1147495 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A0G9R618
Locus tag EGY11_RS05635 Protein ID WP_015369110.1
Coordinates 1146474..1146920 (+) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY11_RS05610 1141995..1142774 + 780 WP_015369105.1 MBL fold metallo-hydrolase -
EGY11_RS05615 1142796..1143749 + 954 WP_032706577.1 helix-turn-helix domain-containing protein -
EGY11_RS05620 1143746..1144795 - 1050 WP_015369107.1 NAD(P)-dependent alcohol dehydrogenase -
EGY11_RS05625 1144953..1145255 - 303 WP_015369108.1 antibiotic biosynthesis monooxygenase -
EGY11_RS05630 1145256..1146263 - 1008 WP_015369109.1 zinc-binding alcohol dehydrogenase family protein -
EGY11_RS05635 1146474..1146920 + 447 WP_015369110.1 helix-turn-helix domain-containing protein Antitoxin
EGY11_RS05640 1146920..1147495 + 576 WP_032706547.1 PIN domain-containing protein Toxin
EGY11_RS05645 1147800..1148996 + 1197 WP_015369112.1 hypothetical protein -
EGY11_RS05650 1149173..1150210 - 1038 WP_032706544.1 virulence RhuM family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-115)

Antitoxin

SinI-like, DNA-binding domain

(79-126)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21688.29 Da        Isoelectric Point: 6.2900

>T114390 WP_032706547.1 NZ_CP033817:1146920-1147495 [Klebsiella aerogenes]
MRHSPYPVILDACVLYPARLRDLLMHLGIAGLYQPKWSRFIHDEWCRNLLINRPDIAPEALRRTVDLMNAALPDANVTGF
AGLINGLVLPDPDDRHVLAAAIRAKAEIIVTLNHKDFPAESLLPFEIETLHPDVFISDLFDLNHALALEAVRRQRQSLKH
PPMTVDEFLEMLLKQGLPMTVKELANYQYAI

Download         Length: 576 bp

>T114390 NZ_CP033817:1146920-1147495 [Klebsiella aerogenes]
ATGCGACATTCACCTTATCCGGTGATTTTAGATGCTTGTGTATTATATCCAGCACGGCTCAGGGATTTATTAATGCACTT
AGGGATCGCTGGACTTTATCAACCAAAATGGAGCCGCTTCATTCACGATGAATGGTGCAGGAATCTTCTTATAAATCGGC
CTGATATAGCACCTGAAGCGCTGCGTCGTACCGTTGATCTCATGAATGCAGCTTTACCGGATGCTAATGTAACGGGTTTT
GCAGGATTAATTAACGGACTAGTTTTACCCGACCCTGACGATCGGCATGTCCTTGCTGCGGCAATCAGAGCGAAGGCTGA
AATTATCGTTACGTTAAATCATAAAGACTTTCCAGCAGAATCATTGCTTCCATTTGAAATCGAAACACTTCATCCTGATG
TATTCATATCGGACCTGTTTGACCTTAATCATGCTCTGGCGTTGGAGGCGGTGAGAAGGCAGCGGCAGTCTCTCAAGCAT
CCTCCAATGACCGTCGATGAGTTCCTGGAGATGCTGCTAAAGCAGGGCTTGCCGATGACGGTAAAAGAGTTAGCCAATTA
CCAGTACGCCATATAG

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16699.26 Da        Isoelectric Point: 6.2257

>AT114390 WP_015369110.1 NZ_CP033817:1146474-1146920 [Klebsiella aerogenes]
MTKLNLPDPLESELAQRGQRELAAYLSTHLETQRIAIVGEDDKTHTIELPTSALTMLMEILGELACGNAVQIVPVHAELT
TQEAANILNVSRPHMVKLLEARKLPFHKTGRHRRVLFADLMEYKKRREQESLDAMQALADQAQDLGMY

Download         Length: 447 bp

>AT114390 NZ_CP033817:1146474-1146920 [Klebsiella aerogenes]
ATGACTAAACTGAACCTTCCGGACCCGCTGGAGAGTGAACTTGCCCAGCGCGGGCAGCGTGAGCTGGCGGCATACCTTTC
TACTCATCTTGAAACGCAGCGTATTGCGATTGTGGGTGAAGATGACAAAACTCATACCATTGAGTTGCCAACCAGCGCTC
TGACTATGCTGATGGAGATCCTTGGGGAATTGGCTTGTGGCAACGCGGTGCAGATTGTTCCTGTTCATGCTGAACTCACC
ACCCAGGAAGCAGCGAATATACTCAATGTTTCTCGTCCGCATATGGTTAAACTGCTGGAAGCGAGGAAGCTTCCATTTCA
TAAAACTGGACGCCATCGCCGGGTTCTGTTTGCCGATCTGATGGAGTATAAAAAACGTAGGGAACAGGAAAGCCTGGACG
CGATGCAAGCGCTGGCGGATCAGGCGCAGGACTTAGGGATGTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9UXE3

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9R618

References