Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4794330..4794972 Replicon chromosome
Accession NZ_CP033795
Organism Bacillus sp. FDAARGOS_527

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EGX95_RS27705 Protein ID WP_000635963.1
Coordinates 4794622..4794972 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EGX95_RS27700 Protein ID WP_000004570.1
Coordinates 4794330..4794617 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX95_RS27675 4789656..4790618 + 963 WP_000961151.1 UV DNA damage repair endonuclease UvsE -
EGX95_RS27680 4790611..4791183 - 573 WP_000906919.1 rhomboid family intramembrane serine protease -
EGX95_RS27685 4791276..4791635 + 360 WP_000635037.1 holo-ACP synthase -
EGX95_RS27690 4791792..4792742 + 951 WP_025991724.1 outer membrane lipoprotein carrier protein LolA -
EGX95_RS27695 4792861..4794030 + 1170 WP_000390601.1 alanine racemase -
EGX95_RS27700 4794330..4794617 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EGX95_RS27705 4794622..4794972 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EGX95_RS27710 4795040..4797208 + 2169 WP_000450602.1 RNA-binding transcriptional accessory protein -
EGX95_RS27715 4797266..4797382 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EGX95_RS27720 4797578..4798036 + 459 WP_000344246.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T114372 WP_000635963.1 NZ_CP033795:4794622-4794972 [Bacillus sp. FDAARGOS_527]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T114372 NZ_CP033795:4794622-4794972 [Bacillus sp. FDAARGOS_527]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT114372 WP_000004570.1 NZ_CP033795:4794330-4794617 [Bacillus sp. FDAARGOS_527]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT114372 NZ_CP033795:4794330-4794617 [Bacillus sp. FDAARGOS_527]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References