Detailed information of TA system TA114367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1088120..1088691
• Replicon: chromosome
• Accession: NZ_CP033787
• Organism: Enterococcus faecalis strain FDAARGOS_528

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EGX75_RS05555
• Protein ID: WP_002354774.1
• Coordinates: 1088350..1088691 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: EGX75_RS05550
• Protein ID: WP_002354773.1
• Coordinates: 1088120..1088350 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX75_RS05530	1084364..1085659	+	1296	WP_002396787.1	AAA family ATPase	-
EGX75_RS05535	1085668..1086300	+	633	WP_002358972.1	RloB family protein	-
EGX75_RS05540	1086367..1087359	-	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
EGX75_RS05545	1087498..1087713	+	216	WP_002358974.1	zinc ribbon domain-containing protein	-
EGX75_RS05550	1088120..1088350	+	231	WP_002354773.1	hypothetical protein	Antitoxin
EGX75_RS05555	1088350..1088691	+	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EGX75_RS05565	1089062..1092676	+	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T114367 WP_002354774.1 NZ_CP033787:1088350-1088691 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T114367 NZ_CP033787:1088350-1088691 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAGTCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT114367 WP_002354773.1 NZ_CP033787:1088120-1088350 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT114367 NZ_CP033787:1088120-1088350 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812