Detailed information of TA system TA114339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 566341..566837
• Replicon: chromosome
• Accession: NZ_CP033787
• Organism: Enterococcus faecalis strain FDAARGOS_528

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3GZA4
• Locus tag: EGX75_RS02830
• Protein ID: WP_002358470.1
• Coordinates: 566341..566610 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R3JPM7
• Locus tag: EGX75_RS02835
• Protein ID: WP_002413218.1
• Coordinates: 566610..566837 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX75_RS02810	563127..563390	-	264	WP_123839658.1	hypothetical protein	-
EGX75_RS02815	563528..563947	-	420	WP_025186145.1	TnpV protein	-
EGX75_RS02820	563973..564686	-	714	WP_002358472.1	hypothetical protein	-
EGX75_RS02825	564694..566274	-	1581	WP_002404807.1	DUF3991 domain-containing protein	-
EGX75_RS02830	566341..566610	-	270	WP_002358470.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX75_RS02835	566610..566837	-	228	WP_002413218.1	antitoxin	Antitoxin
EGX75_RS02840	566897..568132	-	1236	WP_002358469.1	hypothetical protein	-
EGX75_RS02845	568135..568692	-	558	WP_002358242.1	hypothetical protein	-
EGX75_RS02850	568685..568948	-	264	WP_002358468.1	hypothetical protein	-
EGX75_RS02855	569324..570148	-	825	WP_002358467.1	hypothetical protein	-
EGX75_RS02860	570163..570693	-	531	WP_002358466.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	esp / cylI / cylA / cylB / cylM / cylS / cylL / cylR1 / cylR2	499611..567400	67789

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 10.5395

>T114339 WP_002358470.1 NZ_CP033787:c566610-566341 [Enterococcus faecalis]
MSYRLEFTPDVKKQLKKMDKYQATLLTRWLYRNIDGTANPREHGKALSANRAGQWRYRIGNYRVIVEIEDERLVVTAIQV
GHRRNVYDK

Nucleotide

• Length: 270 bp

>T114339 NZ_CP033787:c566610-566341 [Enterococcus faecalis]
ATGTCCTATCGATTAGAATTTACTCCTGATGTAAAAAAACAGTTAAAAAAAATGGATAAATATCAAGCGACATTGCTAAC
AAGATGGTTGTATCGAAATATTGATGGAACGGCCAATCCTAGAGAGCATGGAAAAGCTTTATCAGCAAATCGTGCAGGTC
AGTGGCGATATAGGATAGGGAATTATCGTGTCATTGTAGAAATAGAAGATGAACGTCTTGTTGTTACAGCCATTCAGGTT
GGCCACAGAAGAAATGTTTACGACAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8785.90 Da
• Isoelectric Point: 4.0179

>AT114339 WP_002413218.1 NZ_CP033787:c566837-566610 [Enterococcus faecalis]
MAMITVRVSDEEKEWLSYMAEFFGVTLSDLLKSYSMEQLEDEYDKQTAEIAHKRWIEQGKETISMDEILNEFGGL

Nucleotide

• Length: 228 bp

>AT114339 NZ_CP033787:c566837-566610 [Enterococcus faecalis]
ATGGCTATGATTACTGTAAGAGTGAGTGATGAAGAAAAAGAATGGCTTTCTTACATGGCAGAATTTTTCGGTGTGACGTT
GTCGGATTTGTTAAAAAGCTATTCAATGGAACAATTAGAAGATGAGTACGATAAACAAACAGCAGAAATTGCTCACAAAC
GATGGATTGAGCAAGGAAAAGAAACAATTTCTATGGACGAAATATTAAATGAGTTTGGTGGATTATAA

Structures

Toxin

• AlphaFold DB: R3GZA4

Antitoxin

• AlphaFold DB: R3JPM7