Detailed information of TA system TA114336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 93705..94234
• Replicon: chromosome
• Accession: NZ_CP033782
• Organism: Staphylococcus epidermidis strain FDAARGOS_529

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A939SRT1
• Locus tag: EGX64_RS00640
• Protein ID: WP_002447757.1
• Coordinates: 93705..94067 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5HME6
• Locus tag: EGX64_RS00645
• Protein ID: WP_001829931.1
• Coordinates: 94064..94234 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX64_RS00620	90706..91476	-	771	WP_002447754.1	RNA polymerase sigma factor SigB	-
EGX64_RS00625	91451..91930	-	480	WP_002447755.1	anti-sigma B factor RsbW	-
EGX64_RS00630	91932..92258	-	327	WP_001829952.1	anti-sigma factor antagonist	-
EGX64_RS00635	92358..93359	-	1002	WP_002447756.1	PP2C family protein-serine/threonine phosphatase	-
EGX64_RS00640	93705..94067	-	363	WP_002447757.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EGX64_RS00645	94064..94234	-	171	WP_001829931.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EGX64_RS00650	94321..95469	-	1149	WP_002475027.1	alanine racemase	-
EGX64_RS00655	95536..95889	-	354	WP_002447759.1	holo-ACP synthase	-
EGX64_RS00660	95928..96446	-	519	WP_002447760.1	PH domain-containing protein	-
EGX64_RS00665	96433..97938	-	1506	WP_002447761.1	PH domain-containing protein	-
EGX64_RS00670	97931..98410	-	480	WP_002447762.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13483.62 Da
• Isoelectric Point: 9.9522

>T114336 WP_002447757.1 NZ_CP033782:c94067-93705 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSDSKMIEVDNALDISLGLNNFDHHKS

Nucleotide

• Length: 363 bp

>T114336 NZ_CP033782:c94067-93705 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGATAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGATAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6289.92 Da
• Isoelectric Point: 4.1920

>AT114336 WP_001829931.1 NZ_CP033782:c94234-94064 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Nucleotide

• Length: 171 bp

>AT114336 NZ_CP033782:c94234-94064 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAACGAATCACATTTAATATCTAATTCCA
AGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0N1EF65