Detailed information of TA system TA114335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3552558..3553197
• Replicon: chromosome
• Accession: NZ_CP033780
• Organism: Citrobacter koseri strain FDAARGOS_530

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A381H3Z1
• Locus tag: EGX86_RS16990
• Protein ID: WP_071818852.1
• Coordinates: 3552558..3552734 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EGX86_RS16995
• Protein ID: WP_123907537.1
• Coordinates: 3552781..3553197 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX86_RS16970	3547651..3548526	+	876	WP_012132344.1	AraC family transcriptional regulator	-
EGX86_RS16975	3548503..3549669	-	1167	WP_104868640.1	benzoate/H(+) symporter BenE family transporter	-
EGX86_RS16980	3549762..3550298	+	537	WP_104868641.1	XRE family transcriptional regulator	-
EGX86_RS16985	3550377..3552338	+	1962	WP_114847929.1	U32 family peptidase	-
EGX86_RS16990	3552558..3552734	+	177	WP_071818852.1	type II toxin-antitoxin system HicA family toxin	Toxin
EGX86_RS16995	3552781..3553197	+	417	WP_123907537.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EGX86_RS17000	3553248..3554657	+	1410	WP_114847892.1	PLP-dependent aminotransferase family protein	-
EGX86_RS17005	3554977..3556401	+	1425	WP_012132353.1	aminobutyraldehyde dehydrogenase	-
EGX86_RS17010	3556500..3556595	-	96	WP_071818853.1	stress response membrane protein YncL	-
EGX86_RS17015	3556787..3556960	+	174	WP_049008504.1	GhoT/OrtT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-68)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6810.81 Da
• Isoelectric Point: 10.8122

>T114335 WP_071818852.1 NZ_CP033780:3552558-3552734 [Citrobacter koseri]
VKQSEFRRWLESQGVDVTNGTNHLKLRYHDKRSVMPRHPGDEIKDTLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T114335 NZ_CP033780:3552558-3552734 [Citrobacter koseri]
GTGAAGCAGAGCGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTCGATGTAACAAATGGAACGAATCATTTGAAGTTGAG
ATATCACGACAAACGAAGCGTCATGCCCAGACATCCTGGTGATGAAATTAAAGACACCCTGCGTAAAGCAATACTAAAGC
AGCTTGGTTTGAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15371.57 Da
• Isoelectric Point: 4.4377

>AT114335 WP_123907537.1 NZ_CP033780:3552781-3553197 [Citrobacter koseri]
MRYPVNLIPAEEGGYVVSFPDIPEALTQGDTRHESLEAAQGALVTAFEFYFEDNQPIPLPSMVDEADDYVEIPLSIASKV
LLLNAFLESKITQQELARRIGRPKQEITRLFDLKHATKIDAVQNAARALGKELSLTML

Nucleotide

• Length: 417 bp

>AT114335 NZ_CP033780:3552781-3553197 [Citrobacter koseri]
ATGCGTTATCCCGTCAATCTCATTCCCGCAGAAGAAGGGGGTTACGTTGTGTCGTTCCCGGACATTCCAGAGGCGTTAAC
ACAAGGTGACACCCGCCATGAGTCTCTCGAAGCCGCGCAGGGCGCATTAGTCACCGCCTTTGAATTTTATTTTGAAGATA
ACCAGCCAATTCCTTTACCTTCAATGGTTGATGAAGCTGATGATTATGTGGAGATTCCTCTAAGCATTGCGTCTAAAGTC
CTTTTGTTGAATGCGTTCCTTGAATCAAAAATCACGCAGCAGGAGCTGGCGAGGCGCATTGGCAGACCCAAGCAGGAAAT
TACCCGACTATTCGACTTGAAACACGCAACGAAAATCGATGCTGTACAGAATGCGGCCAGAGCATTGGGTAAAGAGTTAT
CGCTGACTATGCTTTAG

Structures

Toxin

• AlphaFold DB: A0A381H3Z1