Detailed information of TA system TA114325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1086450..1087066
• Replicon: chromosome
• Accession: NZ_CP033780
• Organism: Citrobacter koseri strain FDAARGOS_530

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A8AL49
• Locus tag: EGX86_RS05120
• Protein ID: WP_012133919.1
• Coordinates: 1086450..1086824 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EGX86_RS05125
• Protein ID: WP_162790489.1
• Coordinates: 1086821..1087066 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX86_RS05105	1083953..1084855	+	903	WP_071259455.1	formate dehydrogenase O subunit beta	-
EGX86_RS05110	1084852..1085487	+	636	WP_049011887.1	formate dehydrogenase cytochrome b556 subunit	-
EGX86_RS05115	1085484..1086413	+	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
EGX86_RS05120	1086450..1086824	-	375	WP_012133919.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGX86_RS05125	1086821..1087066	-	246	WP_162790489.1	ribbon-helix-helix domain-containing protein	Antitoxin
EGX86_RS05130	1087302..1088243	-	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
EGX86_RS05135	1088288..1088725	-	438	WP_047459887.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EGX86_RS05140	1088722..1089594	-	873	WP_012133924.1	virulence factor BrkB family protein	-
EGX86_RS05145	1089588..1090187	-	600	WP_047459890.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13741.00 Da
• Isoelectric Point: 9.8061

>T114325 WP_012133919.1 NZ_CP033780:c1086824-1086450 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRGEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T114325 NZ_CP033780:c1086824-1086450 [Citrobacter koseri]
ATGATTAAAGGTCCGGTGTTGTTTGACACCAATATTCTCATTGATTTATTTAGCGGGCGTGGTGAAGCGAAGAAGGCGCT
CGAAGCTTACTCACCCCAGAATGCCATTAGTCTCATTACCTGGATGGAAGTGATGGTAGGGGCAAAAAAGTACCATCAGG
AATCCCGCACACGTATAGCATTGAGCGCTTTTAACGTTATTGGCGTTTCACAGGATATTGCCGAAAGAAGTGTCAGTCTC
AGGCAGGAATACGGGATGAAGTTGCCGGATGCGGTTATTCTGGCTACAGCGCACATTCATCGTTTTGCGCTCGTGACGCG
CAATACAAAGGACTTTGCGGGGATTCCGGGAGTCATTACGCCTTACCATTTATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9524.75 Da
• Isoelectric Point: 4.1961

>AT114325 WP_162790489.1 NZ_CP033780:c1087066-1086821 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW
Q

Nucleotide

• Length: 246 bp

>AT114325 NZ_CP033780:c1087066-1086821 [Citrobacter koseri]
ATGATGGCTGGAATGGAAATGGGCAGAATATTACTTGATTTATCAGATGAGGTTATCAAGCGGCTTGATGACCTCAAACA
ACAGCGTAATCTTCCGCGAGCAGAGCTATTACGCGAAGCAGTTGAACAATACCTGGAAAGGCAGGGACAAGCCGAAACCA
CTATTTCCGATGCGCTGGGGCTCTGGCAAGGTTGCGAAGAAGATGGCGTTGAATACGAAAGGAAGCTCCGCGAGGAGTGG
CAATGA

Structures

Toxin

• AlphaFold DB: A8AL49