Detailed information of TA system TA114324

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 589152..589859
• Replicon: chromosome
• Accession: NZ_CP033780
• Organism: Citrobacter koseri strain FDAARGOS_530

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: EGX86_RS02750
• Protein ID: WP_148084109.1
• Coordinates: 589152..589484 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EGX86_RS02755
• Protein ID: WP_123907366.1
• Coordinates: 589518..589859 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX86_RS02740	585492..586862	-	1371	WP_049010418.1	tyrosine phenol-lyase	-
EGX86_RS02745	587887..588894	-	1008	WP_047041139.1	restriction endonuclease	-
EGX86_RS02750	589152..589484	-	333	WP_148084109.1	TA system toxin CbtA family protein	Toxin
EGX86_RS02755	589518..589859	-	342	WP_123907366.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EGX86_RS02760	589870..590412	-	543	WP_123907367.1	DNA repair protein RadC	-
EGX86_RS02765	590425..590865	-	441	WP_123907368.1	antirestriction protein	-
EGX86_RS02770	590896..591717	-	822	WP_123907369.1	DUF945 domain-containing protein	-
EGX86_RS02775	591838..592311	-	474	WP_123907370.1	hypothetical protein	-
EGX86_RS02780	592383..592835	-	453	WP_000734321.1	hypothetical protein	-
EGX86_RS02785	592871..593587	-	717	WP_123907371.1	WYL domain-containing protein	-
EGX86_RS02790	593831..594706	-	876	WP_123907372.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	587887..623600	35713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (4-106)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12400.24 Da
• Isoelectric Point: 5.6618

>T114324 WP_148084109.1 NZ_CP033780:c589484-589152 [Citrobacter koseri]
MLSTTPQAATLCLSPVAVWQMLLACLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYELVRIDRRGFNWQ
EQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 333 bp

>T114324 NZ_CP033780:c589484-589152 [Citrobacter koseri]
CTGCTGTCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCCGTAGCAGTGTGGCAAATGCTGCTTGCATGCCTGCT
GGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCGATGCCGGTA
TATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGCAGGGGATTCAACTGGCAA
GAACAGTCCCCATATCTGCGGGCAGTAGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAAGAAATCGTATTAG
CGCGGCACAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12754.29 Da
• Isoelectric Point: 6.7482

>AT114324 WP_123907366.1 NZ_CP033780:c589859-589518 [Citrobacter koseri]
MKSTGSESDSFLKWGLNRNVTPFFGARLVQEGNRLHYLADRASISGEFSNAESLKLNEVFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT114324 NZ_CP033780:c589859-589518 [Citrobacter koseri]
ATGAAATCTACTGGCTCAGAGAGCGATTCATTTCTCAAGTGGGGGCTGAATCGTAATGTGACGCCTTTCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTGCACTATCTGGCTGACCGGGCCAGCATTAGCGGTGAATTCAGTAACGCAGAATCTT
TGAAGTTGAATGAAGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCATCGTTAA