114323

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 12702..13322 Replicon chromosome
Accession NZ_CP033780
Organism Citrobacter koseri strain FDAARGOS_530

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EGX86_RS00060 Protein ID WP_002892050.1
Coordinates 13104..13322 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A8AJY4
Locus tag EGX86_RS00055 Protein ID WP_012133514.1
Coordinates 12702..13076 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX86_RS00045 7838..9031 + 1194 WP_012133512.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EGX86_RS00050 9054..12200 + 3147 WP_012133513.1 efflux RND transporter permease AcrB -
EGX86_RS00055 12702..13076 + 375 WP_012133514.1 Hha toxicity modulator TomB Antitoxin
EGX86_RS00060 13104..13322 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EGX86_RS00065 13506..14057 + 552 WP_049009805.1 maltose O-acetyltransferase -
EGX86_RS00070 14171..14641 + 471 WP_114847774.1 hypothetical protein -
EGX86_RS00075 14719..14859 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
EGX86_RS00080 14861..15121 - 261 WP_012133519.1 type B 50S ribosomal protein L31 -
EGX86_RS00085 15347..16897 + 1551 WP_114847716.1 EAL domain-containing protein -
EGX86_RS00090 16937..17290 - 354 WP_012133521.1 DUF1428 family protein -
EGX86_RS00095 17370..17984 - 615 WP_101743908.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T114323 WP_002892050.1 NZ_CP033780:13104-13322 [Citrobacter koseri]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T114323 NZ_CP033780:13104-13322 [Citrobacter koseri]
ATGTCCGATAAACCATTAACGAAAACAGATTATTTGATGCGTTTACGACGCTGCCAGACAATAGACACGCTGGAACGTGT
AATAGAGAAAAATAAATATGAATTGTCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGATCACCGTCTTGCAGAAT
TGACGATGAATAAGCTCTACGACAAGATTCCCACCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14437.27 Da        Isoelectric Point: 5.1444

>AT114323 WP_012133514.1 NZ_CP033780:12702-13076 [Citrobacter koseri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT114323 NZ_CP033780:12702-13076 [Citrobacter koseri]
ATGGATGAGTACTCACCCAAAAGACATGATATCGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCACGGTTGGGTTAATGACCCTACTTCGGCAATCAATCTTCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGCACAGATAGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAATGCGCAGGATCTGCAAAAATGGCGAAAATCAGGAAACCGCCT
GTTTCGCTGCTTCGTCAATGCCACCAGGGCGAATCCTGTTAGCTTGTCATGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A8AJY4

References