Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5386450..5387260 Replicon chromosome
Accession NZ_CP033777
Organism Klebsiella pneumoniae strain FDAARGOS_531

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag EGX99_RS28735 Protein ID WP_004178461.1
Coordinates 5386450..5386983 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag EGX99_RS28740 Protein ID WP_004222378.1
Coordinates 5386994..5387260 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX99_RS28730 5385281..5386402 + 1122 WP_110225560.1 cupin domain-containing protein -
EGX99_RS28735 5386450..5386983 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
EGX99_RS28740 5386994..5387260 - 267 WP_004222378.1 DUF1778 domain-containing protein Antitoxin
EGX99_RS28745 5387363..5388796 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
EGX99_RS28750 5388786..5389469 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
EGX99_RS28760 5389641..5391026 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
EGX99_RS28765 5391044..5391388 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T114322 WP_004178461.1 NZ_CP033777:c5386983-5386450 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T114322 NZ_CP033777:c5386983-5386450 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT114322 WP_004222378.1 NZ_CP033777:c5387260-5386994 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT114322 NZ_CP033777:c5387260-5386994 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAGTGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References