Detailed information of TA system TA114320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3408169..3409757
• Replicon: chromosome
• Accession: NZ_CP033777
• Organism: Klebsiella pneumoniae strain FDAARGOS_531

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: EGX99_RS18965
• Protein ID: WP_032436337.1
• Coordinates: 3408169..3409491 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: EGX99_RS18970
• Protein ID: WP_002904790.1
• Coordinates: 3409491..3409757 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX99_RS18945	3403226..3405727	+	2502	WP_087836359.1	fimbrial biogenesis outer membrane usher protein	-
EGX99_RS18950	3405715..3406719	+	1005	WP_032436338.1	type 1 fimbrial protein	-
EGX99_RS18960	3406860..3408152	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
EGX99_RS18965	3408169..3409491	-	1323	WP_032436337.1	type II toxin-antitoxin system HipA family toxin	Toxin
EGX99_RS18970	3409491..3409757	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
EGX99_RS18975	3410144..3410470	-	327	WP_032436336.1	hydroxyisourate hydrolase	-
EGX99_RS18980	3410467..3410967	-	501	WP_032436335.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
EGX99_RS18985	3410945..3412324	-	1380	Protein_3213	purine permease	-
EGX99_RS18990	3412670..3413824	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
EGX99_RS18995	3413805..3414728	-	924	WP_032436334.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49106.39 Da
• Isoelectric Point: 7.5617

>T114320 WP_032436337.1 NZ_CP033777:c3409491-3408169 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGDHWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T114320 NZ_CP033777:c3409491-3408169 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGATCACTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT114320 WP_002904790.1 NZ_CP033777:c3409757-3409491 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT114320 NZ_CP033777:c3409757-3409491 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTACTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8