Detailed information of TA system TA114296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 420117..421159
• Replicon: chromosome
• Accession: NZ_CP033771
• Organism: Pseudomonas aeruginosa strain FDAARGOS_532

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EGY18_RS01725
• Protein ID: WP_003153636.1
• Coordinates: 420117..420692 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: EGY18_RS01730
• Protein ID: WP_003050245.1
• Coordinates: 420689..421159 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY18_RS01700	416555..417280	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
EGY18_RS01705	417319..418221	-	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
EGY18_RS01710	418221..418721	-	501	WP_003090159.1	hypothetical protein	-
EGY18_RS01715	418718..419188	-	471	WP_003090160.1	hypothetical protein	-
EGY18_RS01720	419185..420099	-	915	WP_016852809.1	AAA family ATPase	-
EGY18_RS01725	420117..420692	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
EGY18_RS01730	420689..421159	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
EGY18_RS01735	421363..421746	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
EGY18_RS01740	421743..421976	+	234	WP_006226027.1	TIGR03758 family integrating conjugative element protein	-
EGY18_RS01745	421993..422352	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
EGY18_RS01750	422364..422762	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
EGY18_RS01755	422759..423451	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
EGY18_RS01760	423448..424359	+	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
EGY18_RS01765	424349..425767	+	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	374001..495733	121732

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T114296 WP_003153636.1 NZ_CP033771:c420692-420117 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T114296 NZ_CP033771:c420692-420117 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT114296 WP_003050245.1 NZ_CP033771:c421159-420689 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT114296 NZ_CP033771:c421159-420689 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68