Detailed information of TA system TA114295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 60324..60912
• Replicon: plasmid unnamed1
• Accession: NZ_CP033769
• Organism: Acinetobacter baumannii strain FDAARGOS_533

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5K1MH32
• Locus tag: EGX83_RS18450
• Protein ID: WP_000569859.1
• Coordinates: 60324..60620 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EGX83_RS18455
• Protein ID: WP_000241337.1
• Coordinates: 60622..60912 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX83_RS18435	56299..57591	+	1293	WP_024436605.1	MFS transporter	-
EGX83_RS18440	57604..58509	+	906	WP_005130397.1	aromatic amino acid DMT transporter YddG	-
EGX83_RS18445	58654..59987	+	1334	Protein_59	ISNCY family transposase	-
EGX83_RS18450	60324..60620	+	297	WP_000569859.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX83_RS18455	60622..60912	+	291	WP_000241337.1	putative addiction module antidote protein	Antitoxin
EGX83_RS18460	61274..62439	+	1166	WP_123794023.1	IS3-like element ISAba22 family transposase	-
EGX83_RS18470	62573..63796	+	1224	WP_123794025.1	IS256 family transposase	-
EGX83_RS18475	63860..64144	-	285	WP_000156168.1	antibiotic biosynthesis monooxygenase	-
EGX83_RS18480	64165..65163	-	999	WP_004740250.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..97783	97783
	-	inside	IScluster/Tn	-	-	58666..63796	5130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11554.35 Da
• Isoelectric Point: 8.5037

>T114295 WP_000569859.1 NZ_CP033769:60324-60620 [Acinetobacter baumannii]
MIEIKRLPEFDEWLDGLKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGAGWRMYYIQHGDVVIVMLGGGEKS
TQKDDIKKAVKLSKTLED

Nucleotide

• Length: 297 bp

>T114295 NZ_CP033769:60324-60620 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTGCCTGAATTTGATGAATGGCTAGATGGTCTTAAAGACAATATGACTCGAATCCGCTTGAA
TCGTCGATTAGATAAGGTCCAACGTGGTAATTGGGGTGACATTAAACCACTCCAAGATGGTGTCTGGGAAATGAGAGAGT
TCTTTGGTGCTGGTTGGCGGATGTACTACATCCAACATGGTGACGTTGTGATCGTGATGCTTGGTGGTGGTGAAAAATCT
ACACAGAAAGATGACATCAAGAAAGCGGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10584.24 Da
• Isoelectric Point: 5.1133

>AT114295 WP_000241337.1 NZ_CP033769:60622-60912 [Acinetobacter baumannii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSTPRFDTI
NRVVNALGLKLTVQHT

Nucleotide

• Length: 291 bp

>AT114295 NZ_CP033769:60622-60912 [Acinetobacter baumannii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGATATTGTAATGTACCTCAA
TATGGTTCTTGAAGAAAATGATCCTGCTGAATTAGCGCACGCACTTGGTGTAATTGCTAAAGCAAGAGGTATGACCCAAA
TTGCGAAAGAAGCAGGTATTGGACGAGAAGCCCTTTACAAAGCACTTCGTCAAGACTCAACGCCTCGTTTTGATACGATC
AACCGTGTTGTGAATGCTTTAGGTTTAAAATTGACAGTTCAACATACTTAA

Structures

Toxin

• AlphaFold DB: A0A5K1MH32