Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4957761..4958379 Replicon chromosome
Accession NZ_CP033762
Organism Escherichia coli strain FDAARGOS_536

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EGX93_RS25305 Protein ID WP_001291435.1
Coordinates 4957761..4957979 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EGX93_RS25310 Protein ID WP_000344800.1
Coordinates 4958005..4958379 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX93_RS25270 4953049..4953621 + 573 WP_000779831.1 YbaY family lipoprotein -
EGX93_RS25275 4953652..4953963 - 312 WP_000409908.1 MGMT family protein -
EGX93_RS25285 4954342..4954695 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EGX93_RS25290 4954736..4956286 - 1551 WP_001357014.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EGX93_RS25295 4956450..4956920 - 471 WP_000136192.1 YlaC family protein -
EGX93_RS25300 4957036..4957587 - 552 WP_024009560.1 maltose O-acetyltransferase -
EGX93_RS25305 4957761..4957979 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EGX93_RS25310 4958005..4958379 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EGX93_RS25315 4958924..4962073 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
EGX93_RS25320 4962096..4963289 - 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T114291 WP_001291435.1 NZ_CP033762:c4957979-4957761 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T114291 NZ_CP033762:c4957979-4957761 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT114291 WP_000344800.1 NZ_CP033762:c4958379-4958005 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT114291 NZ_CP033762:c4958379-4958005 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References