Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4493800..4494350 Replicon chromosome
Accession NZ_CP033762
Organism Escherichia coli strain FDAARGOS_536

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag EGX93_RS23085 Protein ID WP_001160966.1
Coordinates 4494036..4494350 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag EGX93_RS23080 Protein ID WP_000125566.1
Coordinates 4493800..4494033 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX93_RS23055 4488873..4489160 + 288 WP_000203741.1 ferredoxin-like protein FixX -
EGX93_RS23060 4489219..4490550 + 1332 WP_001183207.1 MFS transporter -
EGX93_RS23065 4490658..4491188 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
EGX93_RS23070 4491181..4493043 + 1863 WP_001765385.1 glutathione-regulated potassium-efflux system protein KefC -
EGX93_RS23075 4493235..4493714 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
EGX93_RS23080 4493800..4494033 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
EGX93_RS23085 4494036..4494350 + 315 WP_001160966.1 CcdB family protein Toxin
EGX93_RS23090 4494347..4495195 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
EGX93_RS23095 4495202..4495579 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
EGX93_RS23100 4495582..4496403 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
EGX93_RS23105 4496400..4497389 - 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
EGX93_RS23110 4497389..4498675 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T114289 WP_001160966.1 NZ_CP033762:4494036-4494350 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T114289 NZ_CP033762:4494036-4494350 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT114289 WP_000125566.1 NZ_CP033762:4493800-4494033 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT114289 NZ_CP033762:4493800-4494033 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References