Detailed information of TA system TA114287

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4304194..4305026
• Replicon: chromosome
• Accession: NZ_CP033762
• Organism: Escherichia coli strain FDAARGOS_536

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: EGX93_RS22080
• Protein ID: WP_000854765.1
• Coordinates: 4304652..4305026 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: EGX93_RS22075
• Protein ID: WP_001295723.1
• Coordinates: 4304194..4304562 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX93_RS22040	4301030..4301485	+	456	WP_000581504.1	hypothetical protein	-
EGX93_RS22045	4301564..4301797	+	234	WP_001119729.1	DUF905 family protein	-
EGX93_RS22055	4301897..4302715	+	819	WP_001234620.1	DUF945 domain-containing protein	-
EGX93_RS22060	4302770..4303255	+	486	WP_016237304.1	antirestriction protein	-
EGX93_RS22065	4303271..4303747	+	477	WP_001186773.1	RadC family protein	-
EGX93_RS22070	4303810..4304031	+	222	WP_000692352.1	DUF987 domain-containing protein	-
EGX93_RS22075	4304194..4304562	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EGX93_RS22080	4304652..4305026	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
EGX93_RS22085	4305023..4305514	+	492	WP_000976842.1	hypothetical protein	-
EGX93_RS22090	4305531..4305707	+	177	WP_000839290.1	DUF957 domain-containing protein	-
EGX93_RS25895	4305807..4305965	+	159	WP_001467148.1	hypothetical protein	-
EGX93_RS22100	4306617..4309508	+	2892	WP_000580532.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T114287 WP_000854765.1 NZ_CP033762:4304652-4305026 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T114287 NZ_CP033762:4304652-4305026 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT114287 WP_001295723.1 NZ_CP033762:4304194-4304562 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT114287 NZ_CP033762:4304194-4304562 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52