Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2585456..2586110 Replicon chromosome
Accession NZ_CP033762
Organism Escherichia coli strain FDAARGOS_536

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EGX93_RS13560 Protein ID WP_000244781.1
Coordinates 2585456..2585863 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EGX93_RS13565 Protein ID WP_000354046.1
Coordinates 2585844..2586110 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX93_RS13540 2581413..2583146 - 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -
EGX93_RS13545 2583152..2583862 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EGX93_RS13550 2583887..2584783 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EGX93_RS13555 2584895..2585416 + 522 WP_001055874.1 flavodoxin FldB -
EGX93_RS13560 2585456..2585863 - 408 WP_000244781.1 protein YgfX Toxin
EGX93_RS13565 2585844..2586110 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EGX93_RS13570 2586353..2587333 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
EGX93_RS13575 2587410..2588069 - 660 WP_024946575.1 hemolysin III family protein -
EGX93_RS13580 2588233..2588544 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
EGX93_RS13585 2588589..2590022 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T114280 WP_000244781.1 NZ_CP033762:c2585863-2585456 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T114280 NZ_CP033762:c2585863-2585456 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT114280 WP_000354046.1 NZ_CP033762:c2586110-2585844 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT114280 NZ_CP033762:c2586110-2585844 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References