Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 66257..66816 Replicon plasmid unnamed1
Accession NZ_CP033761
Organism Escherichia coli strain FDAARGOS_536

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag EGX93_RS00395 Protein ID WP_000421272.1
Coordinates 66257..66532 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag EGX93_RS00400 Protein ID WP_001387467.1
Coordinates 66532..66816 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX93_RS00355 62307..63047 - 741 WP_001066953.1 tyrosine-type recombinase/integrase -
EGX93_RS00360 63168..63350 - 183 WP_042344623.1 hypothetical protein -
EGX93_RS00370 63693..64000 - 308 Protein_61 type II toxin-antitoxin system ParD family antitoxin -
EGX93_RS00380 64493..65662 + 1170 WP_001072355.1 hypothetical protein -
EGX93_RS00385 65695..65823 + 129 Protein_63 IS481 family transposase -
EGX93_RS00390 65858..66151 - 294 WP_001105060.1 hypothetical protein -
EGX93_RS00395 66257..66532 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGX93_RS00400 66532..66816 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
EGX93_RS00405 67421..68173 + 753 WP_000562172.1 IclR family transcriptional regulator -
EGX93_RS00410 68219..69184 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
EGX93_RS00415 69217..69597 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
EGX93_RS00420 69622..70512 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
EGX93_RS00425 70745..70939 - 195 WP_000796505.1 hypothetical protein -
EGX93_RS00430 70913..71353 + 441 Protein_72 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / tet(B) iutA / iucD / iucC / iucB / iucA 1..111938 111938


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T114264 WP_000421272.1 NZ_CP033761:c66532-66257 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T114264 NZ_CP033761:c66532-66257 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT114264 WP_001387467.1 NZ_CP033761:c66816-66532 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT114264 NZ_CP033761:c66816-66532 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References