Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 33827..34470 Replicon plasmid unnamed1
Accession NZ_CP033761
Organism Escherichia coli strain FDAARGOS_536

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag EGX93_RS00215 Protein ID WP_001034046.1
Coordinates 34054..34470 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag EGX93_RS00210 Protein ID WP_001261278.1
Coordinates 33827..34057 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX93_RS00180 29338..29643 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
EGX93_RS00185 29645..29863 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
EGX93_RS00190 30430..30942 + 513 WP_000151784.1 hypothetical protein -
EGX93_RS00195 30976..32109 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
EGX93_RS00200 32276..33049 - 774 WP_000905949.1 hypothetical protein -
EGX93_RS00205 33062..33562 - 501 WP_000528931.1 hypothetical protein -
EGX93_RS00210 33827..34057 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EGX93_RS00215 34054..34470 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
EGX93_RS00220 34515..38309 - 3795 WP_001144732.1 hypothetical protein -
EGX93_RS00225 38690..38920 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
EGX93_RS00230 38917..39333 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / tet(B) iutA / iucD / iucC / iucB / iucA 1..111938 111938


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T114262 WP_001034046.1 NZ_CP033761:34054-34470 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T114262 NZ_CP033761:34054-34470 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT114262 WP_001261278.1 NZ_CP033761:33827-34057 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT114262 NZ_CP033761:33827-34057 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References