Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 29338..29863 Replicon plasmid unnamed1
Accession NZ_CP033761
Organism Escherichia coli strain FDAARGOS_536

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag EGX93_RS00180 Protein ID WP_001159868.1
Coordinates 29338..29643 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EGX93_RS00185 Protein ID WP_000813634.1
Coordinates 29645..29863 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX93_RS00165 25248..26414 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
EGX93_RS00170 27002..27757 - 756 WP_000852146.1 replication initiation protein RepE -
EGX93_RS00175 28531..29337 - 807 WP_000016982.1 site-specific integrase -
EGX93_RS00180 29338..29643 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
EGX93_RS00185 29645..29863 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EGX93_RS00190 30430..30942 + 513 WP_000151784.1 hypothetical protein -
EGX93_RS00195 30976..32109 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
EGX93_RS00200 32276..33049 - 774 WP_000905949.1 hypothetical protein -
EGX93_RS00205 33062..33562 - 501 WP_000528931.1 hypothetical protein -
EGX93_RS00210 33827..34057 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
EGX93_RS00215 34054..34470 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / tet(B) iutA / iucD / iucC / iucB / iucA 1..111938 111938


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T114261 WP_001159868.1 NZ_CP033761:c29643-29338 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T114261 NZ_CP033761:c29643-29338 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT114261 WP_000813634.1 NZ_CP033761:c29863-29645 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT114261 NZ_CP033761:c29863-29645 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References