Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 167867..168777 Replicon plasmid unnamed2
Accession NZ_CP033758
Organism Klebsiella pneumoniae strain FDAARGOS_566

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag EGY03_RS28105 Protein ID WP_004144067.1
Coordinates 168307..168777 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag EGY03_RS28100 Protein ID WP_023287184.1
Coordinates 167867..168310 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY03_RS28070 163326..164297 + 972 WP_023287152.1 ParB/RepB/Spo0J family plasmid partition protein -
EGY03_RS28080 165148..165375 - 228 Protein_171 type II toxin-antitoxin system Phd/YefM family antitoxin -
EGY03_RS28085 165454..166085 - 632 Protein_172 DUF4113 domain-containing protein -
EGY03_RS28090 166222..166919 + 698 Protein_173 IS1 family transposase -
EGY03_RS28095 166936..167766 + 831 Protein_174 translesion error-prone DNA polymerase V subunit UmuC -
EGY03_RS28100 167867..168310 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
EGY03_RS28105 168307..168777 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
EGY03_RS28110 168864..169832 + 969 WP_000654811.1 IS5 family transposase -
EGY03_RS28115 169952..170212 - 261 WP_017901409.1 hypothetical protein -
EGY03_RS28120 170913..172282 + 1370 WP_085956500.1 IS3-like element ISKpn8 family transposase -
EGY03_RS28125 172279..173004 - 726 Protein_180 transposase -
EGY03_RS29080 173002..173190 - 189 Protein_181 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid formA - 1..232752 232752
- inside IScluster/Tn - - 166222..174149 7927


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T114260 WP_004144067.1 NZ_CP033758:168307-168777 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T114260 NZ_CP033758:168307-168777 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT114260 WP_023287184.1 NZ_CP033758:167867-168310 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT114260 NZ_CP033758:167867-168310 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAAGTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References