Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3904066..3904685 Replicon chromosome
Accession NZ_CP033756
Organism Klebsiella pneumoniae strain FDAARGOS_566

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EGY03_RS20100 Protein ID WP_002892050.1
Coordinates 3904066..3904284 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EGY03_RS20105 Protein ID WP_002892066.1
Coordinates 3904311..3904685 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY03_RS20070 3900113..3900376 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
EGY03_RS20075 3900376..3900516 + 141 WP_023312743.1 type B 50S ribosomal protein L36 -
EGY03_RS20080 3900513..3901211 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
EGY03_RS20085 3901312..3902763 + 1452 WP_004183206.1 PLP-dependent aminotransferase family protein -
EGY03_RS20090 3902738..3903208 - 471 WP_002892026.1 YlaC family protein -
EGY03_RS28920 3903229..3903369 + 141 WP_032409366.1 hypothetical protein -
EGY03_RS20095 3903341..3903907 - 567 WP_002892030.1 maltose O-acetyltransferase -
EGY03_RS20100 3904066..3904284 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EGY03_RS20105 3904311..3904685 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EGY03_RS20110 3905171..3908317 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
EGY03_RS20115 3908340..3909533 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T114258 WP_002892050.1 NZ_CP033756:c3904284-3904066 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T114258 NZ_CP033756:c3904284-3904066 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT114258 WP_002892066.1 NZ_CP033756:c3904685-3904311 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT114258 NZ_CP033756:c3904685-3904311 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References