Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3245..3770 Replicon plasmid unnamed1
Accession NZ_CP033755
Organism Klebsiella pneumoniae strain FDAARGOS_566

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag EGY03_RS00035 Protein ID WP_025714517.1
Coordinates 3465..3770 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag EGY03_RS00030 Protein ID WP_001568025.1
Coordinates 3245..3463 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY03_RS00010 927..1955 - 1029 WP_001568023.1 Abi family protein -
EGY03_RS00015 2096..2689 - 594 WP_123806157.1 hypothetical protein -
EGY03_RS00030 3245..3463 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EGY03_RS00035 3465..3770 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
EGY03_RS00040 3939..4334 + 396 WP_017899885.1 hypothetical protein -
EGY03_RS00045 4361..4675 + 315 WP_053389906.1 hypothetical protein -
EGY03_RS00050 4686..5702 + 1017 WP_017899884.1 hypothetical protein -
EGY03_RS00055 5900..6694 + 795 WP_004197635.1 site-specific integrase -
EGY03_RS28540 7174..7476 - 303 WP_071571079.1 hypothetical protein -
EGY03_RS00065 7473..8099 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..103852 103852


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T114240 WP_025714517.1 NZ_CP033755:3465-3770 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T114240 NZ_CP033755:3465-3770 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT114240 WP_001568025.1 NZ_CP033755:3245-3463 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT114240 NZ_CP033755:3245-3463 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References