Detailed information of TA system TA114234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 3052628..3053265
• Replicon: chromosome
• Accession: NZ_CP033750
• Organism: Burkholderia multivorans strain FDAARGOS_547

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EGY20_RS28860
• Protein ID: WP_035953036.1
• Coordinates: 3052864..3053265 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: J5BG47
• Locus tag: EGY20_RS28855
• Protein ID: WP_006415629.1
• Coordinates: 3052628..3052864 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY20_RS28830	3047797..3048339	+	543	WP_123791032.1	hypothetical protein	-
EGY20_RS28840	3048501..3049532	+	1032	WP_012212492.1	helix-turn-helix domain-containing protein	-
EGY20_RS28845	3049651..3050739	-	1089	WP_123791033.1	ADP-heptose--LPS heptosyltransferase	-
EGY20_RS28850	3050736..3052439	-	1704	WP_123791034.1	thiamine pyrophosphate-binding protein	-
EGY20_RS28855	3052628..3052864	+	237	WP_006415629.1	hypothetical protein	Antitoxin
EGY20_RS28860	3052864..3053265	+	402	WP_035953036.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGY20_RS28865	3053387..3054775	-	1389	WP_006401425.1	L-serine ammonia-lyase	-
EGY20_RS28870	3054802..3055944	-	1143	WP_006401424.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14907.07 Da
• Isoelectric Point: 5.7429

>T114234 WP_035953036.1 NZ_CP033750:3052864-3053265 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTVCANPARERRNLAALIEDIPVAPFDGAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFAGYPGLRLENWLTG

Nucleotide

• Length: 402 bp

>T114234 NZ_CP033750:3052864-3053265 [Burkholderia multivorans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTATCTGATGAAGAACCAGCCGGAGCAGGTCGCGCGGCGTTTCGC
GCAGTGCTACACGGGGGACGTCGTCATGTCGGCGATCACCTATGCGGAACTCGAGTACGGCGTGACGGTATGTGCAAATC
CGGCCCGCGAACGCCGCAATCTCGCCGCGCTGATCGAGGATATCCCGGTTGCGCCGTTCGACGGAGCGGCTGCGCAGGCA
TACGGCCCCATTCGCGAGGCGACGCGGGAACGCAAGAAGGATCATCTCGACAAGCTGATCGGCGCGCATGCGGTATCGCT
CGATGTCGTGCTGGTGACCAACAACGAGCGTGATTTCGCGGGTTATCCGGGCTTGAGGCTCGAGAACTGGCTGACCGGCT
AG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8973.04 Da
• Isoelectric Point: 5.1651

>AT114234 WP_006415629.1 NZ_CP033750:3052628-3052864 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREDW

Nucleotide

• Length: 237 bp

>AT114234 NZ_CP033750:3052628-3052864 [Burkholderia multivorans]
ATGCATACCACCCGGGTCTTCCGGAATGGCAACTCGCAGGCGGTGCGGATTCCTGCAGATCTGGCTTATGAGCGCAGCGA
TATCGAACTCGAAATCGAGCGTGTCGGTGATGAAATTCGCATCCGGCCTGCGCGGCGGCCGCTGACCGGCGTGCTCGGCA
AGTTCGCGAAATTCGGGCCGGATTTCATGGCCGAGGGGCGCGGCGATCAGGAACAGGCCGAGCGCGAGGATTGGTGA

Structures

Antitoxin

• AlphaFold DB: J5BG47