Detailed information of TA system TA114232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1032326..1032901
• Replicon: chromosome
• Accession: NZ_CP033750
• Organism: Burkholderia multivorans strain FDAARGOS_547

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EGY20_RS18875
• Protein ID: WP_123790428.1
• Coordinates: 1032614..1032901 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J4SGI3
• Locus tag: EGY20_RS18870
• Protein ID: WP_006409266.1
• Coordinates: 1032326..1032610 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY20_RS18845	1027959..1029380	-	1422	WP_060146402.1	FAD-binding oxidoreductase	-
EGY20_RS18850	1029399..1029806	-	408	WP_123790427.1	DUF2069 domain-containing protein	-
EGY20_RS18855	1029803..1030414	-	612	WP_006402099.1	NAD(P)H:quinone oxidoreductase	-
EGY20_RS18860	1030559..1031887	+	1329	WP_039224705.1	YihY family inner membrane protein	-
EGY20_RS18865	1032054..1032242	-	189	WP_123791126.1	hypothetical protein	-
EGY20_RS18870	1032326..1032610	-	285	WP_006409266.1	putative addiction module antidote protein	Antitoxin
EGY20_RS18875	1032614..1032901	-	288	WP_123790428.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY20_RS18880	1033114..1033431	-	318	WP_006402097.1	DUF962 domain-containing protein	-
EGY20_RS18885	1033592..1034047	+	456	WP_006402096.1	CBS domain-containing protein	-
EGY20_RS18890	1034195..1036129	+	1935	WP_105790217.1	MFS transporter	-
EGY20_RS18895	1036298..1037398	+	1101	WP_048804937.1	chorismate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (6-94)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10957.62 Da
• Isoelectric Point: 9.7646

>T114232 WP_123790428.1 NZ_CP033750:c1032901-1032614 [Burkholderia multivorans]
MLRSTEFNDWLANLRDQRGKARIAARLISAQLGNFGEYRVLDEGVCELKIDFGPGYRVYYVRRGEVVYLLLCGGDKSTQR
KDIKRAAQMARELKE

Nucleotide

• Length: 288 bp

>T114232 NZ_CP033750:c1032901-1032614 [Burkholderia multivorans]
TTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCGCAGCCCGCCT
GATCAGCGCGCAGCTCGGCAACTTCGGCGAGTACCGTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCGATTTCGGTC
CGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCAACGCAACGG
AAGGACATCAAGCGCGCCGCGCAGATGGCGCGCGAACTGAAGGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9986.38 Da
• Isoelectric Point: 6.2121

>AT114232 WP_006409266.1 NZ_CP033750:c1032610-1032326 [Burkholderia multivorans]
MTNYTEFDAADYLDNDEAVAEFLAVAAEDPNPDVFLAAVAAVARARGMTKVAAAAGVGRVALHKALTPGAHPRYETVQKI
IRSLGLRMSFTVAH

Nucleotide

• Length: 285 bp

>AT114232 NZ_CP033750:c1032610-1032326 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGATCCGAATCCCGACGTGTTCCTCGCGGCCGTCGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAAGTTGCGGCAG
CCGCCGGCGTCGGCCGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGTTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Structures

Antitoxin

• AlphaFold DB: J4SGI3