Detailed information of TA system TA114226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 718675..719295
• Replicon: chromosome
• Accession: NZ_CP033748
• Organism: Burkholderia multivorans strain FDAARGOS_547

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EGY20_RS03380
• Protein ID: WP_105764778.1
• Coordinates: 718978..719295 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: B9B269
• Locus tag: EGY20_RS03375
• Protein ID: WP_006398012.1
• Coordinates: 718675..718974 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY20_RS03350	713907..715178	+	1272	WP_006412648.1	Rieske 2Fe-2S domain-containing protein	-
EGY20_RS03355	715193..715681	+	489	WP_006398008.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
EGY20_RS03360	715699..716025	+	327	WP_006404219.1	non-heme iron oxygenase ferredoxin subunit	-
EGY20_RS03365	716015..717235	+	1221	WP_123789007.1	FAD-dependent oxidoreductase	-
EGY20_RS03370	717349..718635	+	1287	WP_123789008.1	DUF445 domain-containing protein	-
EGY20_RS03375	718675..718974	-	300	WP_006398012.1	putative addiction module antidote protein	Antitoxin
EGY20_RS03380	718978..719295	-	318	WP_105764778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY20_RS03390	719658..721298	+	1641	WP_006398015.1	acetolactate synthase large subunit	-
EGY20_RS03395	721327..722760	+	1434	WP_059843122.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-89)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12096.92 Da
• Isoelectric Point: 10.2349

>T114226 WP_105764778.1 NZ_CP033748:c719295-718978 [Burkholderia multivorans]
MPYLAPTFSIRTTAVFDAWFAGLQDRTAKRRVQARIDRLSMGNLGNWKSVGSPVYEMRIDHGPGYRVYFVRRRTIWVVLL
CGGDKSTQRDDIRAAHAMLEHLDLE

Nucleotide

• Length: 318 bp

>T114226 NZ_CP033748:c719295-718978 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCGTTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGGCGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10605.00 Da
• Isoelectric Point: 6.6381

>AT114226 WP_006398012.1 NZ_CP033748:c718974-718675 [Burkholderia multivorans]
MSKISTRPWDSAEHLKTDDDMAEYFEACLQEGGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT114226 NZ_CP033748:c718974-718675 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
CTGCCTCCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3KTI5