Detailed information of TA system TA114224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2115574..2116265
• Replicon: chromosome
• Accession: NZ_CP033747
• Organism: Burkholderia multivorans strain FDAARGOS_548

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EGY19_RS30555
• Protein ID: WP_105772970.1
• Coordinates: 2115574..2116029 (-)
• Length: 152 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A132F1C7
• Locus tag: EGY19_RS30560
• Protein ID: WP_012216644.1
• Coordinates: 2116026..2116265 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY19_RS30535	2111991..2112941	+	951	WP_105773815.1	DMT family transporter	-
EGY19_RS30540	2113145..2113720	+	576	WP_105773814.1	permease	-
EGY19_RS30545	2113814..2113948	-	135	WP_006396654.1	entericidin A/B family lipoprotein	-
EGY19_RS30550	2114181..2115476	+	1296	WP_012216646.1	aspartate carbamoyltransferase	-
EGY19_RS30555	2115574..2116029	-	456	WP_105772970.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGY19_RS30560	2116026..2116265	-	240	WP_012216644.1	Arc family DNA-binding protein	Antitoxin
EGY19_RS30565	2116570..2118816	+	2247	WP_105772971.1	TonB-dependent siderophore receptor	-
EGY19_RS30570	2118820..2119575	+	756	WP_048804528.1	sel1 repeat family protein	-
EGY19_RS30575	2119572..2120255	+	684	WP_105772972.1	Fe2+-dependent dioxygenase	-
EGY19_RS30580	2120661..2120900	+	240	WP_006416205.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2110027..2111286	1259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 152 a.a.
• Molecular weight: 16223.63 Da
• Isoelectric Point: 7.2060

>T114224 WP_105772970.1 NZ_CP033747:c2116029-2115574 [Burkholderia multivorans]
MILVDTNVIAEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFAVAALPEGRRRDWLAQSIEHRVVPVFRGRILPFDD
AASKAYARIRAHARAGGNAIAPADGYIAATAEANGLIVATRDVALFEAAGLRVIDPWASQGAPVRQGIASK

Nucleotide

• Length: 456 bp

>T114224 NZ_CP033747:c2116029-2115574 [Burkholderia multivorans]
ATGATCCTTGTTGATACGAACGTCATTGCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
ACAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGCTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGCGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGAGCAAGGCCTATGCGCGCATTCGCGCGCACGCCCGTGCCGGCGGCAACGCGATCGCGCCCGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCTGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGTGGGCGTCGCAGGGCGCGCCGGTGCGCCAAGGTATCGCGTCGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8716.94 Da
• Isoelectric Point: 5.1133

>AT114224 WP_012216644.1 NZ_CP033747:c2116265-2116026 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPMSFE

Nucleotide

• Length: 240 bp

>AT114224 NZ_CP033747:c2116265-2116026 [Burkholderia multivorans]
ATGCCGGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCAGCGCTGCACGGGCGCAG
TACCGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTCGGAACCTTGCTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATTCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A132F1C7