Detailed information of TA system TA114221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 892420..893040
• Replicon: chromosome
• Accession: NZ_CP033747
• Organism: Burkholderia multivorans strain FDAARGOS_548

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EGY19_RS24950
• Protein ID: WP_105771373.1
• Coordinates: 892723..893040 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: B9B269
• Locus tag: EGY19_RS24945
• Protein ID: WP_006398012.1
• Coordinates: 892420..892719 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY19_RS24920	887657..888928	+	1272	WP_059875252.1	Rieske 2Fe-2S domain-containing protein	-
EGY19_RS24925	888943..889431	+	489	WP_006398008.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
EGY19_RS24930	889449..889775	+	327	WP_006404219.1	non-heme iron oxygenase ferredoxin subunit	-
EGY19_RS24935	889765..890985	+	1221	WP_105771372.1	FAD-dependent oxidoreductase	-
EGY19_RS24940	891099..892385	+	1287	WP_039225729.1	DUF445 domain-containing protein	-
EGY19_RS24945	892420..892719	-	300	WP_006398012.1	putative addiction module antidote protein	Antitoxin
EGY19_RS24950	892723..893040	-	318	WP_105771373.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY19_RS24960	893403..895043	+	1641	WP_006398015.1	acetolactate synthase large subunit	-
EGY19_RS24965	895072..896505	+	1434	WP_105771374.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (9-101)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12168.98 Da
• Isoelectric Point: 9.9414

>T114221 WP_105771373.1 NZ_CP033747:c893040-892723 [Burkholderia multivorans]
MPYLAPTFSIRTTAVFDAWFAGLQDRTAKRRIQARIDRLSMGNLGNWKSVGSPVYEMRIDHGPGYRVYFVRRRTIWVVLL
CDGDKSTQRDDIRAAHAMLEHLDLE

Nucleotide

• Length: 318 bp

>T114221 NZ_CP033747:c893040-892723 [Burkholderia multivorans]
ATGCCTTACCTTGCTCCAACGTTCAGCATCCGGACGACCGCCGTGTTCGACGCGTGGTTTGCCGGCCTGCAGGACCGCAC
CGCGAAGCGCCGCATTCAGGCGCGCATCGATCGGCTGTCGATGGGCAATCTGGGCAACTGGAAGTCCGTCGGGTCGCCCG
TCTACGAGATGCGCATCGATCACGGCCCCGGCTATCGCGTGTACTTCGTGCGGCGGCGCACGATATGGGTCGTCCTGCTG
TGCGACGGCGACAAATCCACGCAGCGCGACGACATTCGCGCCGCCCATGCGATGCTCGAGCATCTCGACCTGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10605.00 Da
• Isoelectric Point: 6.6381

>AT114221 WP_006398012.1 NZ_CP033747:c892719-892420 [Burkholderia multivorans]
MSKISTRPWDSAEHLKTDDDMAEYFEACLQEGGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT114221 NZ_CP033747:c892719-892420 [Burkholderia multivorans]
ATGAGCAAGATCAGCACGCGGCCGTGGGATTCGGCCGAACACCTGAAGACCGACGACGACATGGCCGAGTATTTCGAAGC
CTGCCTGCAGGAAGGCGGCGACGATCCGGCATTCATCGCGCACGCGCTCGGCGTGATCGCGCGCGCCCGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGGCTATCGCGCGAGGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTGATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCACGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3KTI5