Detailed information of TA system TA114220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 232009..232604
• Replicon: chromosome
• Accession: NZ_CP033747
• Organism: Burkholderia multivorans strain FDAARGOS_548

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: EGY19_RS21855
• Protein ID: WP_105772925.1
• Coordinates: 232254..232604 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A2S9MLU5
• Locus tag: EGY19_RS21850
• Protein ID: WP_059843544.1
• Coordinates: 232009..232260 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY19_RS21835	227871..228842	-	972	WP_012216344.1	LysR family transcriptional regulator	-
EGY19_RS21840	228984..230288	+	1305	WP_059843537.1	MFS transporter	-
EGY19_RS21845	230388..231881	+	1494	WP_105772926.1	M81 family metallopeptidase	-
EGY19_RS21850	232009..232260	+	252	WP_059843544.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EGY19_RS21855	232254..232604	+	351	WP_105772925.1	type II toxin-antitoxin system ChpB family toxin	Toxin
EGY19_RS21860	232783..234318	-	1536	WP_039223192.1	multidrug efflux transporter outer membrane subunit OpcM	-
EGY19_RS21865	234329..237514	-	3186	WP_006412982.1	multidrug efflux RND transporter permease subunit CeoB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12370.33 Da
• Isoelectric Point: 8.8909

>T114220 WP_105772925.1 NZ_CP033747:232254-232604 [Burkholderia multivorans]
MVKRVKFERGDIVRVSPNPTLGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGAGTETQGVALV
NMARMLDLEARGARKVERAPVEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T114220 NZ_CP033747:232254-232604 [Burkholderia multivorans]
ATGGTGAAGCGCGTCAAGTTCGAGCGAGGCGATATCGTGCGCGTGAGCCCGAATCCGACTCTCGGTCGTGAGCAGCAGGG
CGACTTTCGTCCGGCGCTGGTGCTCTCCCCGGCAGCGTTCAACGCTTTGGGCGTTGCGCTGGTCGCACCGATCACCCAGG
GTGGCGAATTTGCGCGGTTCGCAGGGTTCGCGGTCCCGCTGTCCGGTGCAGGAACCGAGACGCAGGGCGTCGCGCTGGTG
AACATGGCTCGCATGCTCGACCTGGAGGCGCGCGGCGCTCGCAAGGTCGAACGCGCGCCTGTCGAGGTCGTGGAAGATGC
ACTCGCCCGGCTTCAGACGATCATCGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9021.38 Da
• Isoelectric Point: 6.2518

>AT114220 WP_059843544.1 NZ_CP033747:232009-232260 [Burkholderia multivorans]
MELKIQKWGNSAAVRLPSVLLEQINATVGSSLSADVRPDGVLLTPARRKYSLDELVAQCDTKAPFPADMAAWGEVKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT114220 NZ_CP033747:232009-232260 [Burkholderia multivorans]
ATGGAACTCAAGATTCAAAAATGGGGCAATAGTGCGGCGGTTCGCTTGCCCAGCGTTCTGCTCGAACAGATCAACGCGAC
GGTCGGCAGTTCGCTGAGCGCGGACGTGCGCCCGGATGGCGTGTTGTTGACGCCGGCCCGCCGCAAGTATTCGCTCGATG
AGCTCGTTGCCCAATGCGACACGAAAGCGCCTTTCCCTGCCGACATGGCTGCATGGGGCGAGGTCAAGCCGGTGGGGCGT
GAGGCATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9MLU5