Detailed information of TA system TA114218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1258861..1259445
• Replicon: chromosome
• Accession: NZ_CP033745
• Organism: Burkholderia multivorans strain FDAARGOS_548

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EGY19_RS06150
• Protein ID: WP_105772250.1
• Coordinates: 1259149..1259445 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J4SGI3
• Locus tag: EGY19_RS06145
• Protein ID: WP_006409266.1
• Coordinates: 1258861..1259145 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY19_RS06120	1254497..1255918	-	1422	WP_060152585.1	FAD-binding oxidoreductase	-
EGY19_RS06125	1255937..1256341	-	405	WP_039224708.1	DUF2069 domain-containing protein	-
EGY19_RS06130	1256338..1256949	-	612	WP_105772249.1	NAD(P)H:quinone oxidoreductase	-
EGY19_RS06135	1257094..1258422	+	1329	WP_039224705.1	YihY family inner membrane protein	-
EGY19_RS06140	1258589..1258777	-	189	WP_060152588.1	hypothetical protein	-
EGY19_RS06145	1258861..1259145	-	285	WP_006409266.1	putative addiction module antidote protein	Antitoxin
EGY19_RS06150	1259149..1259445	-	297	WP_105772250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY19_RS06155	1259651..1259968	-	318	WP_006402097.1	DUF962 domain-containing protein	-
EGY19_RS06160	1260129..1260584	+	456	WP_105857520.1	CBS domain-containing protein	-
EGY19_RS06165	1260733..1262667	+	1935	WP_105772252.1	MFS transporter	-
EGY19_RS06170	1262836..1263936	+	1101	WP_006413771.1	chorismate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11240.00 Da
• Isoelectric Point: 9.4615

>T114218 WP_105772250.1 NZ_CP033745:c1259445-1259149 [Burkholderia multivorans]
MVTLLRSTEFNDWLANLRDQRGKARIAARLISAQLGNFGEYPVLDEGVCELKIDFGPGYRVYYVRRGEVVYLLLCGGDKS
TQRKDIKRAVQMARELKE

Nucleotide

• Length: 297 bp

>T114218 NZ_CP033745:c1259445-1259149 [Burkholderia multivorans]
ATGGTTACTTTGCTGCGATCCACGGAGTTCAACGATTGGCTTGCCAACCTGCGCGATCAGCGCGGCAAGGCCCGAATCGC
AGCCCGCCTGATCAGCGCGCAGCTCGGCAACTTCGGCGAGTACCCTGTGCTCGACGAAGGGGTCTGCGAACTGAAGATCG
ATTTCGGTCCGGGCTACCGCGTGTATTACGTTCGACGCGGCGAAGTGGTCTACCTCCTTCTGTGCGGCGGCGACAAGTCA
ACGCAACGGAAGGACATCAAGCGCGCCGTACAGATGGCGCGCGAACTGAAGGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9986.38 Da
• Isoelectric Point: 6.2121

>AT114218 WP_006409266.1 NZ_CP033745:c1259145-1258861 [Burkholderia multivorans]
MTNYTEFDAADYLDNDEAVAEFLAVAAEDPNPDVFLAAVAAVARARGMTKVAAAAGVGRVALHKALTPGAHPRYETVQKI
IRSLGLRMSFTVAH

Nucleotide

• Length: 285 bp

>AT114218 NZ_CP033745:c1259145-1258861 [Burkholderia multivorans]
ATGACCAACTACACCGAGTTCGATGCGGCCGATTACCTCGACAACGATGAGGCGGTCGCGGAATTCCTCGCCGTCGCGGC
AGAGGACCCGAATCCTGACGTGTTCCTCGCGGCGGTTGCTGCGGTCGCGCGGGCGCGCGGTATGACGAAGGTTGCAGCAG
CAGCCGGCGTCGGCCGCGTCGCGCTTCACAAGGCACTGACGCCCGGCGCGCACCCGCGCTACGAAACCGTGCAGAAAATC
ATCCGGTCGCTCGGCCTGCGCATGAGCTTCACGGTCGCGCACTAA

Structures

Antitoxin

• AlphaFold DB: J4SGI3