Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3985448..3986068 Replicon chromosome
Accession NZ_CP033744
Organism Citrobacter freundii strain FDAARGOS_549

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EGX89_RS19940 Protein ID WP_002892050.1
Coordinates 3985448..3985666 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag EGX89_RS19945 Protein ID WP_003021733.1
Coordinates 3985694..3986068 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX89_RS19905 3980807..3981436 + 630 WP_016149709.1 membrane protein -
EGX89_RS19910 3981501..3981854 + 354 WP_003831033.1 DUF1428 family protein -
EGX89_RS19915 3981906..3983459 - 1554 WP_003835927.1 EAL domain-containing protein -
EGX89_RS19920 3983648..3983908 + 261 WP_003021719.1 type B 50S ribosomal protein L31 -
EGX89_RS19925 3983910..3984050 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
EGX89_RS19930 3984128..3984598 - 471 WP_003021724.1 YlaC family protein -
EGX89_RS19935 3984715..3985266 - 552 WP_003835924.1 maltose O-acetyltransferase -
EGX89_RS19940 3985448..3985666 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EGX89_RS19945 3985694..3986068 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
EGX89_RS19950 3986557..3989706 - 3150 WP_123924888.1 efflux RND transporter permease AcrB -
EGX89_RS19955 3989729..3990922 - 1194 WP_088902653.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T114216 WP_002892050.1 NZ_CP033744:c3985666-3985448 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T114216 NZ_CP033744:c3985666-3985448 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATCCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT114216 WP_003021733.1 NZ_CP033744:c3986068-3985694 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT114216 NZ_CP033744:c3986068-3985694 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References