Detailed information of TA system TA114215

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3783950..3784656
• Replicon: chromosome
• Accession: NZ_CP033744
• Organism: Citrobacter freundii strain FDAARGOS_549

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: EGX89_RS19005
• Protein ID: WP_039556156.1
• Coordinates: 3784288..3784656 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EGX89_RS19000
• Protein ID: WP_039556157.1
• Coordinates: 3783950..3784267 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX89_RS18965	3780050..3780766	+	717	WP_058647035.1	WYL domain-containing protein	-
EGX89_RS18970	3780802..3781254	+	453	WP_089586936.1	hypothetical protein	-
EGX89_RS18975	3781326..3781799	+	474	WP_058647034.1	hypothetical protein	-
EGX89_RS18980	3781919..3782740	+	822	WP_058647033.1	DUF945 domain-containing protein	-
EGX89_RS18985	3782771..3783214	+	444	WP_096164809.1	antirestriction protein	-
EGX89_RS18990	3783223..3783705	+	483	WP_039556158.1	DNA repair protein RadC	-
EGX89_RS18995	3783714..3783931	+	218	Protein_3488	DUF987 family protein	-
EGX89_RS19000	3783950..3784267	+	318	WP_039556157.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EGX89_RS19005	3784288..3784656	+	369	WP_039556156.1	TA system toxin CbtA family protein	Toxin
EGX89_RS19015	3785228..3786706	+	1479	WP_121571462.1	hypothetical protein	-
EGX89_RS19020	3786872..3788053	-	1182	WP_121571463.1	pyridoxal phosphate-dependent aminotransferase	-
EGX89_RS19025	3788066..3789517	-	1452	WP_003838742.1	Na+/H+ antiporter NhaC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3783714..3792885	9171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13715.99 Da
• Isoelectric Point: 8.7174

>T114215 WP_039556156.1 NZ_CP033744:3784288-3784656 [Citrobacter freundii]
MKTLPATISRATKPCLSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETIIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T114215 NZ_CP033744:3784288-3784656 [Citrobacter freundii]
ATGAAAACTCTACCTGCAACAATTTCGCGGGCGACGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGATGCTAAGCGACACACCTTTTAGTGATGAAACCATTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATCGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGCTGATGAGTCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGTCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11901.43 Da
• Isoelectric Point: 6.6246

>AT114215 WP_039556157.1 NZ_CP033744:3783950-3784267 [Citrobacter freundii]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASITGKFSDAECRKLDETFPHFISQMESMLTTGEMNPRHAHCVILYH
NGFTCEADTLGSCGYVYISVYPTQR

Nucleotide

• Length: 318 bp

>AT114215 NZ_CP033744:3783950-3784267 [Citrobacter freundii]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAAGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAAATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGCGTCATCCTGTATCAC
AACGGTTTCACCTGCGAAGCCGACACTCTTGGCAGTTGCGGCTATGTATACATCTCCGTCTATCCCACTCAACGCTAA