Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2848434..2849050 Replicon chromosome
Accession NZ_CP033744
Organism Citrobacter freundii strain FDAARGOS_549

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag EGX89_RS14625 Protein ID WP_003028682.1
Coordinates 2848676..2849050 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag EGX89_RS14620 Protein ID WP_043018956.1
Coordinates 2848434..2848676 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX89_RS14590 2844284..2844883 + 600 WP_016151258.1 glucose-1-phosphatase -
EGX89_RS14595 2844877..2845749 + 873 WP_003028669.1 virulence factor BrkB family protein -
EGX89_RS14600 2845746..2846183 + 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
EGX89_RS14605 2846228..2847169 + 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
EGX89_RS14615 2847320..2848228 - 909 WP_071687352.1 alpha/beta hydrolase -
EGX89_RS14620 2848434..2848676 + 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
EGX89_RS14625 2848676..2849050 + 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
EGX89_RS14630 2849087..2850016 - 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
EGX89_RS14635 2850013..2850648 - 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
EGX89_RS14640 2850645..2851547 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T114213 WP_003028682.1 NZ_CP033744:2848676-2849050 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T114213 NZ_CP033744:2848676-2849050 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT114213 WP_043018956.1 NZ_CP033744:2848434-2848676 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT114213 NZ_CP033744:2848434-2848676 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References