Detailed information of TA system TA114203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 198778..199417
• Replicon: chromosome
• Accession: NZ_CP033744
• Organism: Citrobacter freundii strain FDAARGOS_549

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: EGX89_RS01605
• Protein ID: WP_003020221.1
• Coordinates: 199241..199417 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EGX89_RS01600
• Protein ID: WP_123924900.1
• Coordinates: 198778..199194 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX89_RS01580	193824..194768	-	945	WP_003843727.1	ABC transporter permease	-
EGX89_RS01585	194769..195785	-	1017	WP_121571759.1	ABC transporter ATP-binding protein	-
EGX89_RS01590	195802..196947	-	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
EGX89_RS01595	197276..198685	-	1410	WP_044702348.1	PLP-dependent aminotransferase family protein	-
EGX89_RS01600	198778..199194	-	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EGX89_RS01605	199241..199417	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
EGX89_RS01610	199681..201084	+	1404	WP_121571760.1	cytochrome ubiquinol oxidase subunit I	-
EGX89_RS01615	201084..202094	+	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
EGX89_RS01620	202094..202219	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
EGX89_RS01625	202409..202639	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T114203 WP_003020221.1 NZ_CP033744:c199417-199241 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T114203 NZ_CP033744:c199417-199241 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT114203 WP_123924900.1 NZ_CP033744:c199194-198778 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT114203 NZ_CP033744:c199194-198778 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCAGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36